| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1521 | Methanosphaera | TGTGACGACTGGGGAATGC | TGCTACTCCTGCTCCACCT | 60.00 | 59.92 | 285 | 44 |
100.00%
|
33.33%
|
| Primer 1522 | Methanosphaera | ACCACAAAGAAAAGTACGCCT | TCGTCCTCCTACTCCTTGGA | 58.07 | 59.00 | 166 | 44 |
100.00%
|
33.33%
|
| Primer 1523 | Methanosphaera | ACCACAAAGAAAAGTACGCCT | AGTGCTGATACTCGTCCTCCT | 58.07 | 60.06 | 177 | 44 |
100.00%
|
33.33%
|
| Primer 1524 | Methanosphaera | ATGCAGGTGAATGGAGGGG | CCTCCAGGCCAACGTGTTA | 59.38 | 59.63 | 187 | 44 |
100.00%
|
66.67%
|
| Primer 1525 | Methanosphaera | GCAGGTGAATGGAGGGGAA | CCTCCAGGCCAACGTGTTA | 59.31 | 59.63 | 185 | 44 |
100.00%
|
66.67%
|
| Primer 1526 | Methanosphaera | AGCCCAACAACCAGACCTA | AGGTTCACCTGTACGTGCTA | 58.14 | 58.38 | 226 | 44 |
100.00%
|
66.67%
|
| Primer 1527 | Methanosphaera | CCAGGGGTAAATCGGCAGTT | AGCCTCATGTGCTGCTCT | 60.03 | 58.60 | 194 | 44 |
100.00%
|
66.67%
|
| Primer 1528 | Methanosphaera | AGATTACCACCAACATCCCCT | AGCAGCTGCAACACCAGT | 58.43 | 59.81 | 156 | 44 |
100.00%
|
66.67%
|
| Primer 1529 | Methanosphaera | AGATTACCACCAACATCCCCT | AGCAGCTGCAACACCAGTA | 58.43 | 59.55 | 156 | 44 |
100.00%
|
66.67%
|
| Primer 1530 | Methanosphaera | TGACTTCATGGGAGCACGAG | CCTTCACGTATCCGGTAGTCA | 59.75 | 58.98 | 186 | 43 |
100.00%
|
33.33%
|