| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1541 | Methanosphaera | AATTGCAATAGGACTTGGCGA | TGCTCGGAAGATGTGTGGA | 58.28 | 58.64 | 202 | 42 |
100.00%
|
33.33%
|
| Primer 1542 | Methanosphaera | ATTTGGACGTGGAGGCGA | TGGTGTGTCAGCATCACGA | 58.93 | 59.25 | 292 | 42 |
100.00%
|
33.33%
|
| Primer 1543 | Methanosphaera | TTTGGACGTGGAGGCGAAG | TGGTGTGTCAGCATCACGA | 60.30 | 59.25 | 291 | 42 |
100.00%
|
33.33%
|
| Primer 1544 | Methanosphaera | ATTTGGACGTGGAGGCGAA | TGGTGTGTCAGCATCACGA | 59.63 | 59.25 | 292 | 42 |
100.00%
|
33.33%
|
| Primer 1545 | Methanosphaera | TTGCAGGAGGGCTCTTTGC | AGCTGCATCTGCGAAACGA | 60.60 | 60.37 | 167 | 42 |
100.00%
|
66.67%
|
| Primer 1546 | Methanosphaera | CAGGAGGGCTCTTTGCATCA | GCTGCATCTGCGAAACGA | 60.03 | 58.83 | 163 | 42 |
100.00%
|
66.67%
|
| Primer 1547 | Methanosphaera | TGCATGACAGTGGCCTAAATG | TCCTGGTGCTTTAGGTGCAA | 58.91 | 59.52 | 296 | 41 |
100.00%
|
66.67%
|
| Primer 1548 | Methanosphaera | AGCCCAACAACCAGACCT | AGGTTCACCTGTACGTGCTAT | 58.33 | 58.82 | 226 | 41 |
100.00%
|
66.67%
|
| Primer 1549 | Methanosphaera | CCATGTCCTCCAACAGTGCT | ACGTCTTGCAGCCCAACAT | 59.96 | 60.23 | 282 | 39 |
100.00%
|
33.33%
|
| Primer 1550 | Methanosphaera | ACTTGGAGCAGCACTTGTTG | TGCTGCCCATGAAGCTATGAA | 58.97 | 60.06 | 235 | 39 |
100.00%
|
33.33%
|