| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1551 | Methanosphaera | TTTGGACGTGGAGGCGAA | CTGGTGTGTCAGCATCACG | 59.18 | 58.84 | 292 | 39 |
100.00%
|
33.33%
|
| Primer 1552 | Methanosphaera | ACATGGAGGAGCAGCTGGT | AGCTGCACCCCAGTAGAAAT | 60.92 | 59.00 | 235 | 39 |
100.00%
|
33.33%
|
| Primer 1553 | Methanosphaera | CCAACCCCTTTACAGGTGGA | TGGTGGTTGGGTGTAGGGT | 59.23 | 60.39 | 230 | 39 |
100.00%
|
66.67%
|
| Primer 1554 | Methanosphaera | TCCAACCCCTTTACAGGTGG | TGGTGGTTGGGTGTAGGGT | 59.23 | 60.39 | 231 | 39 |
100.00%
|
66.67%
|
| Primer 1555 | Methanosphaera | GTTGGATGTGGAACTGGGG | AGGTTACCTCCACTTCCACC | 58.04 | 58.64 | 221 | 37 |
100.00%
|
66.67%
|
| Primer 1556 | Methanosphaera | ATGTTGGATGTGGAACTGGGG | GGTTACCTCCACTTCCACCA | 60.27 | 58.94 | 222 | 37 |
100.00%
|
66.67%
|
| Primer 1557 | Methanosphaera | GCAAACAACTCTGGAGCAACA | AGAGTCCTGTATCCCCTGCA | 59.59 | 59.66 | 259 | 37 |
100.00%
|
66.67%
|
| Primer 1558 | Methanosphaera | CCCATATCAACAGCTGAAGGC | TGGTATCTTGCCTCATGGGG | 59.05 | 59.15 | 182 | 37 |
100.00%
|
66.67%
|
| Primer 1559 | Methanosphaera | GGTCCAACACCTCTTGCATCT | TTGTATTTAGAAGCGCAGGGG | 60.27 | 58.36 | 178 | 37 |
100.00%
|
66.67%
|
| Primer 1560 | Methanosphaera | TGGTGATGCCACATCAGTTCA | TCTGTTGAACCCCTACCAAGA | 59.93 | 58.30 | 297 | 36 |
100.00%
|
66.67%
|