| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 291 | Methanosphaera | CCGGTGCACAGGACTTTACA | ATGCCTGCTAGAAGTCCTGT | 60.25 | 58.42 | 198 | 104 |
100.00%
|
33.33%
|
| Primer 292 | Methanosphaera | AGGTGGAGCAAACGCTGT | GCATTCCCCAGTCGTCACA | 59.49 | 60.00 | 272 | 104 |
100.00%
|
33.33%
|
| Primer 293 | Methanosphaera | GTGACAATGGCAGTATGGGC | ACAGCTCCTGGTGCTAGAAA | 59.26 | 58.65 | 208 | 104 |
100.00%
|
33.33%
|
| Primer 294 | Methanosphaera | TCTGTAAGTGCATGGGCTCC | TCTCCACGTTCTGGTGTGT | 59.75 | 58.49 | 170 | 104 |
100.00%
|
33.33%
|
| Primer 295 | Methanosphaera | TGTAGCATGCGCAAGTTCAC | TCTCCACGTTCTGGTGTGT | 59.48 | 58.49 | 195 | 104 |
100.00%
|
33.33%
|
| Primer 296 | Methanosphaera | TGTTGGATGTGGAACTGGGG | GGTTACCTCCACTTCCACCA | 59.89 | 58.94 | 221 | 104 |
100.00%
|
66.67%
|
| Primer 297 | Methanosphaera | TGGGATGATGGTGCTGGAG | GTCCCCACCAACCATTTGC | 59.08 | 59.33 | 291 | 104 |
100.00%
|
66.67%
|
| Primer 298 | Methanosphaera | CCATGAACACAAACCCCAACA | CACATGGTGCTGGTGTTCC | 59.24 | 59.04 | 239 | 104 |
100.00%
|
66.67%
|
| Primer 299 | Methanosphaera | CCATGAACACAAACCCCAACA | CCACATGGTGCTGGTGTTC | 59.24 | 59.04 | 240 | 104 |
100.00%
|
66.67%
|
| Primer 300 | Methanosphaera | CCATGAACACAAACCCCAACA | GGTGTTCCTACAGAGGGTGC | 59.24 | 60.04 | 228 | 104 |
100.00%
|
66.67%
|