| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 301 | Methanosphaera | AGTAGTAGGTGTGGGCATGT | CCTTTTGTAGGCGTGCTGTC | 58.05 | 59.48 | 219 | 104 |
100.00%
|
66.67%
|
| Primer 302 | Methanosphaera | AACCCAATGGCTACCGGTG | TGAACGAGGTCTGCTCCTT | 60.00 | 58.25 | 227 | 103 |
100.00%
|
33.33%
|
| Primer 303 | Methanosphaera | ACCCTGAACAACTCCGCTT | AGGACATGTTGCTACAGCCA | 59.16 | 59.31 | 176 | 103 |
100.00%
|
33.33%
|
| Primer 304 | Methanosphaera | TGCAGGAGTCGGTGACATG | GGCCAGAGATCTGCAGCTT | 59.71 | 59.78 | 228 | 103 |
100.00%
|
33.33%
|
| Primer 305 | Methanosphaera | GTCGAGGTAAAGCAGCAGGT | GCAAGTGTCCATGTAGCGC | 60.04 | 59.57 | 160 | 103 |
100.00%
|
66.67%
|
| Primer 306 | Methanosphaera | TCGAGGTAAAGCAGCAGGTG | GCAAGTGTCCATGTAGCGC | 60.04 | 59.57 | 159 | 103 |
100.00%
|
66.67%
|
| Primer 307 | Methanosphaera | TGTCGAGGTAAAGCAGCAGG | GCAAGTGTCCATGTAGCGC | 60.04 | 59.57 | 161 | 103 |
100.00%
|
66.67%
|
| Primer 308 | Methanosphaera | TGCTGTGCATTTTCAACCAGG | AGCAAGAACCAGGGCTGTC | 59.93 | 59.93 | 280 | 102 |
100.00%
|
33.33%
|
| Primer 309 | Methanosphaera | GTCCTGCACTTGGAGCTGT | TCTTTTGCTCCTTCTTGGCG | 59.93 | 58.76 | 265 | 102 |
100.00%
|
33.33%
|
| Primer 310 | Methanosphaera | CCAACAAAACACCCATCCCG | TGCACGTCCGTGTGTTGT | 59.68 | 60.13 | 228 | 102 |
100.00%
|
33.33%
|