| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 401 | Methanosphaera | TGCAGCAATGGAACTACCGG | GGGTCTCTATGCCATAGTGGT | 60.68 | 58.67 | 216 | 95 |
100.00%
|
33.33%
|
| Primer 402 | Methanosphaera | GCAGCAATGGAACTACCGGT | GGGTCTCTATGCCATAGTGGT | 60.68 | 58.67 | 215 | 95 |
100.00%
|
33.33%
|
| Primer 403 | Methanosphaera | ACCAGGGGAACGTGCAAT | TCCACAGCATTTTCCGCAG | 59.16 | 58.74 | 264 | 95 |
100.00%
|
66.67%
|
| Primer 404 | Methanosphaera | AGCCCAACAACCAGACCTAAT | AGGTTCACCTGTACGTGCT | 59.29 | 58.56 | 226 | 95 |
100.00%
|
66.67%
|
| Primer 405 | Methanosphaera | AGGGAAAAGAGAAGTGGGGT | TTGATACCTCCTGAAGCGGAT | 58.17 | 58.60 | 223 | 95 |
100.00%
|
66.67%
|
| Primer 406 | Methanosphaera | AGGGAAAAGAGAAGTGGGGT | ATTGATACCTCCTGAAGCGGA | 58.17 | 58.60 | 224 | 95 |
100.00%
|
66.67%
|
| Primer 407 | Methanosphaera | AGGGAAAAGAGAAGTGGGGT | CATTGATACCTCCTGAAGCGG | 58.17 | 58.51 | 225 | 95 |
100.00%
|
66.67%
|
| Primer 408 | Methanosphaera | TGGGGATAGTGGTTGTGGAA | AGGCCAACGTCTCATCCA | 58.25 | 58.19 | 259 | 95 |
100.00%
|
66.67%
|
| Primer 409 | Methanosphaera | TTGGGGATAGTGGTTGTGGAA | AGGCCAACGTCTCATCCA | 58.93 | 58.19 | 260 | 95 |
100.00%
|
66.67%
|
| Primer 410 | Methanosphaera | CTTGCTTCAGCTGGAGTAGGT | TGCTGGCCAAAGATCTGCA | 59.72 | 59.92 | 240 | 95 |
100.00%
|
66.67%
|