| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 421 | Methanosphaera | TCCCCAATCCCGAAGAGCT | TGCTCCACCACAATCATTGCT | 60.31 | 60.55 | 154 | 95 |
100.00%
|
66.67%
|
| Primer 422 | Methanosphaera | TCGTGATTGGGCAGCAGT | CTGCAGCAATCACATGTGGT | 59.25 | 59.11 | 215 | 95 |
100.00%
|
66.67%
|
| Primer 423 | Methanosphaera | TGTTCGTCGTGATTGGGCA | CTGCAGCAATCACATGTGGT | 59.93 | 59.11 | 221 | 95 |
100.00%
|
66.67%
|
| Primer 424 | Methanosphaera | AGACAACCATTCCCAGGGC | TGGCCATGGAATGTCTGGT | 59.62 | 58.91 | 276 | 95 |
100.00%
|
66.67%
|
| Primer 425 | Methanosphaera | AGCAGCACACCCGTATTGT | CACTCGCTCCAATCTCAGGAA | 59.63 | 59.79 | 167 | 95 |
100.00%
|
66.67%
|
| Primer 426 | Methanosphaera | GACTCGGTCCTCAACTTGCA | AGCAAATGGTCCCCAAGCA | 59.97 | 59.84 | 188 | 94 |
100.00%
|
33.33%
|
| Primer 427 | Methanosphaera | GACTCGGTCCTCAACTTGCA | TGGTCCCCAAGCAACCAT | 59.97 | 58.73 | 182 | 94 |
100.00%
|
33.33%
|
| Primer 428 | Methanosphaera | GCAGCAGCAGTAGCATCAC | CACGGCTTAGTGGTGGTTCT | 59.28 | 59.97 | 175 | 94 |
100.00%
|
33.33%
|
| Primer 429 | Methanosphaera | GCAGCAGCAGTAGCATCAC | ACGGCTTAGTGGTGGTTCT | 59.28 | 58.55 | 174 | 94 |
100.00%
|
33.33%
|
| Primer 430 | Methanosphaera | AAAGTGGGGTAGGACATGCA | TGCTGCTTCAAATGTAGCACC | 58.93 | 59.46 | 239 | 94 |
100.00%
|
33.33%
|