| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 501 | Methanosphaera | AAAGGATGGCCATCTGGTAGA | GCTCTTGGAGTCCAACCTCT | 58.51 | 59.02 | 170 | 92 |
100.00%
|
100.00%
|
| Primer 502 | Methanosphaera | AAAGGATGGCCATCTGGTAGA | ATGCTCTTGGAGTCCAACCTC | 58.51 | 59.72 | 172 | 92 |
100.00%
|
100.00%
|
| Primer 503 | Methanosphaera | TGGAACAGCAGCTGCACT | CTTGATCTGGCCATGTTGCTT | 59.49 | 59.18 | 251 | 92 |
100.00%
|
150.00%
|
| Primer 504 | Methanosphaera | TGGAACAGCAGCTGCACT | TTGATCTGGCCATGTTGCTT | 59.49 | 58.07 | 250 | 92 |
100.00%
|
150.00%
|
| Primer 505 | Methanosphaera | AGATGCCCATGTACATGCAGA | ACACCATGTTTTGCCACACG | 59.51 | 59.90 | 183 | 92 |
100.00%
|
100.00%
|
| Primer 506 | Methanosphaera | TCCCCAATCCCGAAGAGCT | GCTCCACCACAATCATTGCTT | 60.31 | 59.45 | 153 | 92 |
100.00%
|
100.00%
|
| Primer 507 | Methanosphaera | GACGTGGTGAAATTCCAGGAG | AATTTGCTTCTGTACGGGTGT | 58.92 | 58.14 | 219 | 92 |
100.00%
|
100.00%
|
| Primer 508 | Methanosphaera | CGAGGTAAAGCAGCAGGTGT | GCAAGTGTCCATGTAGCGC | 60.32 | 59.57 | 158 | 92 |
100.00%
|
100.00%
|
| Primer 509 | Methanosphaera | TCGAGGTAAAGCAGCAGGT | GCAAGTGTCCATGTAGCGC | 58.64 | 59.57 | 159 | 92 |
100.00%
|
100.00%
|
| Primer 510 | Methanosphaera | GGAGCAGCATTTTGTCGAGG | GCAAGTGTCCATGTAGCGC | 59.55 | 59.57 | 173 | 92 |
100.00%
|
150.00%
|