| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 521 | Methanosphaera | TCACACGAGCTGCAGACA | GGTGCTGCTATTACAAGGCG | 58.87 | 59.34 | 151 | 91 |
100.00%
|
33.33%
|
| Primer 522 | Methanosphaera | AGGGGAAGTCAGCTTGCAC | GCAGCGATGGTAAGTTGGTC | 59.93 | 59.27 | 257 | 91 |
100.00%
|
33.33%
|
| Primer 523 | Methanosphaera | AGGAACAGGAACCAAGCCC | TGGAGGTGTTGAATGTCCCA | 59.54 | 58.86 | 160 | 91 |
100.00%
|
33.33%
|
| Primer 524 | Methanosphaera | AGGAACAGGAACCAAGCCC | GTGGAGGTGTTGAATGTCCCA | 59.54 | 60.20 | 161 | 91 |
100.00%
|
33.33%
|
| Primer 525 | Methanosphaera | GCTCTCTCCCGTGATATTGCA | AACTTGAAGGTAGAGCTCCCA | 59.93 | 58.38 | 271 | 91 |
100.00%
|
66.67%
|
| Primer 526 | Methanosphaera | TGCTCTCTCCCGTGATATTGC | AACTTGAAGGTAGAGCTCCCA | 59.93 | 58.38 | 272 | 91 |
100.00%
|
66.67%
|
| Primer 527 | Methanosphaera | ATGGATACGGCTGGAGCAC | GACGGACAGTTCCATGCCA | 59.55 | 60.00 | 208 | 91 |
100.00%
|
66.67%
|
| Primer 528 | Methanosphaera | ATGGATACGGCTGGAGCAC | ACGGACAGTTCCATGCCA | 59.55 | 58.84 | 207 | 91 |
100.00%
|
66.67%
|
| Primer 529 | Methanosphaera | AGCACTACTATTTGCAGCCG | CTGGTCGTGGTGTATGTTTGT | 58.34 | 58.51 | 179 | 91 |
100.00%
|
66.67%
|
| Primer 530 | Methanosphaera | GTTCCTGGACCAAGAGGAGG | TGCTTCACAATCCCCTGGTC | 59.38 | 59.96 | 177 | 91 |
100.00%
|
66.67%
|