| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 541 | Methanosphaera | TGATGAACCAACAAGCGGACT | TGTGCTATCCATGGAAGACGT | 60.20 | 59.17 | 246 | 90 |
100.00%
|
50.00%
|
| Primer 542 | Methanosphaera | ACCAACAAGCGGACTAGATCC | TGTGCTATCCATGGAAGACGT | 59.79 | 59.17 | 240 | 90 |
100.00%
|
50.00%
|
| Primer 543 | Methanosphaera | ACGCCAACAGCAGATATGC | AGCATCTCCACGTGTAGCAC | 58.60 | 60.11 | 285 | 90 |
100.00%
|
50.00%
|
| Primer 544 | Methanosphaera | ACTGGGCAGATGTATCAGCAC | AAGTGCTGTAACGCGTGC | 60.13 | 59.06 | 164 | 90 |
100.00%
|
50.00%
|
| Primer 545 | Methanosphaera | ACCCTGTTAAAGCAGCTGC | GGTGTGTTTGAAGGGCTTGC | 58.35 | 60.25 | 178 | 90 |
100.00%
|
50.00%
|
| Primer 546 | Methanosphaera | ACCCTGTTAAAGCAGCTGC | GGGCTTGCTCTACCAAATGC | 58.35 | 59.54 | 166 | 90 |
100.00%
|
50.00%
|
| Primer 547 | Methanosphaera | ATGTGTTGCGGTGCAGGT | AGTCCCATTGCTAGTCCGT | 60.20 | 58.01 | 236 | 90 |
100.00%
|
50.00%
|
| Primer 548 | Methanosphaera | TGGGGACTTCCACTTCTTGC | ACTCCAGCACCACCAACAC | 59.89 | 60.15 | 272 | 90 |
100.00%
|
100.00%
|
| Primer 549 | Methanosphaera | TTGCAGTGCCTGGATGTCC | CACGAGTAGGGCATGTTGC | 60.30 | 58.91 | 204 | 90 |
100.00%
|
100.00%
|
| Primer 550 | Methanosphaera | AAACAAGTGCTGCCTGTGC | TCCCGTAGATATTCCAGCACC | 59.56 | 59.03 | 182 | 90 |
100.00%
|
100.00%
|