| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 691 | Methanosphaera | TCACGCGTTGTAGATAGAGCT | ATAGCTCCAAGGCATGCCG | 58.99 | 60.23 | 155 | 84 |
100.00%
|
66.67%
|
| Primer 692 | Methanosphaera | TGCCATAGCAGGAGTATACCC | CCCCAGGTGTTGAAACTTCC | 58.74 | 58.67 | 290 | 84 |
100.00%
|
66.67%
|
| Primer 693 | Methanosphaera | TGGCTGCTGTTGCTGGAA | TGAGGAGGTGCATATGCCG | 59.80 | 59.55 | 215 | 84 |
100.00%
|
66.67%
|
| Primer 694 | Methanosphaera | AGACGTGGTGAAATTCCAGGA | AATTTGCTTCTGTACGGGTGT | 59.30 | 58.14 | 220 | 84 |
100.00%
|
66.67%
|
| Primer 695 | Methanosphaera | ACTGCATGGACTCAGAAGGA | TGGCCTTACTCAGTGGAGGT | 58.35 | 60.18 | 160 | 84 |
100.00%
|
66.67%
|
| Primer 696 | Methanosphaera | GCAGCCCAAGAATTTGCTCT | GCTTCATCTTGCATTTGGGCA | 59.11 | 60.07 | 158 | 83 |
100.00%
|
33.33%
|
| Primer 697 | Methanosphaera | GCAGCCCAAGAATTTGCTCT | TGCTTCATCTTGCATTTGGGC | 59.11 | 60.07 | 159 | 83 |
100.00%
|
33.33%
|
| Primer 698 | Methanosphaera | TGCAGCACTAGGAAAACGTGA | TCGCGTCTGGTGGTTTCT | 60.20 | 58.87 | 261 | 83 |
100.00%
|
33.33%
|
| Primer 699 | Methanosphaera | TGCAGCACTAGGAAAACGTG | TCGCGTCTGGTGGTTTCT | 58.77 | 58.87 | 261 | 83 |
100.00%
|
33.33%
|
| Primer 700 | Methanosphaera | TCGTGCTGAAGATGCTTGTAG | ATCGTGACCACCCCTTTCT | 58.39 | 58.23 | 220 | 83 |
100.00%
|
66.67%
|