| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 701 | Methanosphaera | TGCAATTGGTGAATGTGGTCT | GTTCATCACAGATCCATCCGG | 58.40 | 58.51 | 281 | 83 |
100.00%
|
66.67%
|
| Primer 702 | Methanosphaera | TGCAATTGGTGAATGTGGTCT | TCATCACAGATCCATCCGGTA | 58.40 | 58.04 | 279 | 83 |
100.00%
|
66.67%
|
| Primer 703 | Methanosphaera | AGGGCTGTTGCAACAAGTCT | GCACAAACTGGCGTGTCTG | 60.11 | 60.01 | 179 | 83 |
100.00%
|
66.67%
|
| Primer 704 | Methanosphaera | AGGGCTGTTGCAACAAGTCT | TGCACAAACTGGCGTGTC | 60.11 | 58.89 | 180 | 83 |
100.00%
|
66.67%
|
| Primer 705 | Methanosphaera | ACTCGGTCCTCAACTTGCA | AGCAAATGGTCCCCAAGCA | 58.87 | 59.84 | 187 | 82 |
100.00%
|
33.33%
|
| Primer 706 | Methanosphaera | ACTCGGTCCTCAACTTGCA | TGGTCCCCAAGCAACCAT | 58.87 | 58.73 | 181 | 82 |
100.00%
|
33.33%
|
| Primer 707 | Methanosphaera | AGACTCGGTCCTCAACTTGC | AGCAAATGGTCCCCAAGCA | 59.68 | 59.84 | 189 | 82 |
100.00%
|
33.33%
|
| Primer 708 | Methanosphaera | AGGAGCAGGAATTGGAACACT | AGTGCATCGCCTTTGATTAGT | 59.29 | 58.01 | 153 | 82 |
100.00%
|
33.33%
|
| Primer 709 | Methanosphaera | CTTGTAAGACGAGACTGGGCA | GCTGCTATTACGTGTGGTGC | 59.73 | 59.63 | 221 | 82 |
100.00%
|
33.33%
|
| Primer 710 | Methanosphaera | TTGTAAGACGAGACTGGGCA | GCTGCTATTACGTGTGGTGC | 58.67 | 59.63 | 220 | 82 |
100.00%
|
33.33%
|