| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 911 | Methanosphaera | ACTGAAGCTGTACGTGCCG | ACAGCCATAACACCTGCTTGA | 60.37 | 59.93 | 209 | 76 |
100.00%
|
66.67%
|
| Primer 912 | Methanosphaera | GTGGCACGAGGAGCAACATA | ACCGGTAAGATAAGCAGCAGA | 60.39 | 58.89 | 258 | 75 |
100.00%
|
66.67%
|
| Primer 913 | Methanosphaera | TGGCACGAGGAGCAACATAT | ACCGGTAAGATAAGCAGCAGA | 59.46 | 58.89 | 257 | 75 |
100.00%
|
66.67%
|
| Primer 914 | Methanosphaera | TGGAACAACCGCAAGTTCAA | GCTTCAACTCCAGGACTAGCA | 58.54 | 59.72 | 168 | 74 |
100.00%
|
33.33%
|
| Primer 915 | Methanosphaera | TGGAACAACCGCAAGTTCAA | TGCTTCAACTCCAGGACTAGC | 58.54 | 59.72 | 169 | 74 |
100.00%
|
33.33%
|
| Primer 916 | Methanosphaera | TCATGGGTCCGGTTGCAA | ACCCAGTGCTACTCCAAATGA | 59.16 | 59.01 | 257 | 74 |
100.00%
|
33.33%
|
| Primer 917 | Methanosphaera | ATCATGGGTCCGGTTGCAA | ACCCAGTGCTACTCCAAATGA | 59.62 | 59.01 | 258 | 74 |
100.00%
|
33.33%
|
| Primer 918 | Methanosphaera | GCAGCAATGGAACTACCGG | AGGGTCTCTATGCCATAGTGG | 58.90 | 58.39 | 216 | 74 |
100.00%
|
33.33%
|
| Primer 919 | Methanosphaera | TGCAGCAATGGAACTACCGG | AGGGTCTCTATGCCATAGTGG | 60.68 | 58.39 | 217 | 74 |
100.00%
|
33.33%
|
| Primer 920 | Methanosphaera | ACCACATGCATGTACTGGTGA | TGCCCTCAGCTTCCACAA | 59.65 | 58.75 | 182 | 74 |
100.00%
|
33.33%
|