| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 931 | Methanosphaera | TGGGGATAGTGGTTGTGGAAA | AGGCCAACGTCTCATCCAA | 58.93 | 58.93 | 259 | 74 |
100.00%
|
66.67%
|
| Primer 932 | Methanosphaera | AAAGGCGACATCACCGAGG | ATTGCTGCTCCTCTACCGG | 60.08 | 59.17 | 182 | 74 |
100.00%
|
66.67%
|
| Primer 933 | Methanosphaera | GGCGACATCACCGAGGAAA | ATTGCTGCTCCTCTACCGG | 60.08 | 59.17 | 179 | 74 |
100.00%
|
66.67%
|
| Primer 934 | Methanosphaera | TGGCTGTGATCCTAAGGCTG | ACACCCCTACCAGCACAA | 59.46 | 58.00 | 186 | 74 |
100.00%
|
66.67%
|
| Primer 935 | Methanosphaera | ACCTGTCACACCAGCAAGT | TTTGAGCTACTCCAACCGGA | 59.08 | 58.66 | 245 | 73 |
100.00%
|
33.33%
|
| Primer 936 | Methanosphaera | TCCTTCATGGGCTTTTCAGGA | GCAAGTTTCAGATTTCCTGGC | 59.29 | 58.05 | 186 | 73 |
100.00%
|
33.33%
|
| Primer 937 | Methanosphaera | TGGGCTTTTCAGGAATTTGGA | GCAAGTTTCAGATTTCCTGGC | 58.03 | 58.05 | 179 | 73 |
100.00%
|
33.33%
|
| Primer 938 | Methanosphaera | TCAGCACGCGTAATAACAGGA | CCAAGAAGTGCACCTAGTGGA | 59.80 | 59.65 | 242 | 73 |
100.00%
|
33.33%
|
| Primer 939 | Methanosphaera | TCAGCACGCGTAATAACAGGA | ACAAACACCAAGAAGTGCACC | 59.80 | 59.52 | 249 | 73 |
100.00%
|
33.33%
|
| Primer 940 | Methanosphaera | AGCATCAAACTGGGCTGC | GGGTCAGTTGGTGTTTCTGGA | 58.31 | 60.13 | 216 | 73 |
100.00%
|
33.33%
|