| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Methylococcus | TTTTCACCCGAGCCGTGT | TTTCAGCCAGGCCATCAGG | 59.49 | 60.00 | 183 | 148 |
100.00%
|
100.00%
|
| Primer 2 | Methylococcus | CGCAAAACGCAGCACTGT | GCCGTGTTTCAACAGCAGG | 59.98 | 60.01 | 190 | 148 |
100.00%
|
100.00%
|
| Primer 3 | Methylococcus | ACATCGCCAAGGTCGAAGC | TTGGAGATCCGCAGACTGC | 60.74 | 59.78 | 154 | 146 |
100.00%
|
100.00%
|
| Primer 4 | Methylococcus | GTCCTTTTCTGGCTGGCAC | TTGGAGATCCGCAGACTGC | 59.04 | 59.78 | 212 | 146 |
100.00%
|
100.00%
|
| Primer 5 | Methylococcus | CCTTTTCTGGCTGGCACTG | TTGGAGATCCGCAGACTGC | 59.04 | 59.78 | 210 | 146 |
100.00%
|
100.00%
|
| Primer 6 | Methylococcus | AACATCGTGCAGCGCAGT | AGCTCACGCTGTTGCAGT | 60.67 | 59.89 | 169 | 143 |
100.00%
|
100.00%
|
| Primer 7 | Methylococcus | ACATCGTGCAGCGCAGT | AGCTCACGCTGTTGCAGT | 60.01 | 59.89 | 168 | 143 |
100.00%
|
100.00%
|
| Primer 8 | Methylococcus | ATTTCCCTCACCCTTCGGC | AGCTCACGCTGTTGCAGT | 59.70 | 59.89 | 207 | 143 |
100.00%
|
100.00%
|
| Primer 9 | Methylococcus | TGCGCATCATGTTGCTGG | TCGAATCCGACACCAGCTG | 59.12 | 59.78 | 186 | 141 |
100.00%
|
100.00%
|
| Primer 10 | Methylococcus | TGATGGAAGCCATGCGCA | ACCGACTCCTTCTCCGGAT | 60.05 | 59.69 | 166 | 138 |
100.00%
|
100.00%
|