| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 91 | Methylorubrum | TCAACCATCCCCACGCT | GTCGCGACATGGGCAATCA | 58.04 | 61.11 | 206 | 110 |
100.00%
|
50.00%
|
| Primer 92 | Methylorubrum | TCAACCATCCCCACGCT | CGCGACATGGGCAATCA | 58.04 | 58.06 | 204 | 110 |
100.00%
|
50.00%
|
| Primer 93 | Methylorubrum | TCAACCATCCCCACGCT | GTCGCGACATGGGCAAT | 58.04 | 58.06 | 206 | 110 |
100.00%
|
50.00%
|
| Primer 94 | Methylorubrum | TTCATGGCCTTGCTCGGA | GCGTGGTAGTGCAGGTTCT | 58.92 | 60.00 | 212 | 109 |
100.00%
|
25.00%
|
| Primer 95 | Methylorubrum | CTTCATGGCCTTGCTCGGA | GCGTGGTAGTGCAGGTTCT | 60.08 | 60.00 | 213 | 109 |
100.00%
|
25.00%
|
| Primer 96 | Methylorubrum | TTCATGGCCTTGCTCGGAC | GCGTGGTAGTGCAGGTTCT | 60.38 | 60.00 | 212 | 109 |
100.00%
|
25.00%
|
| Primer 97 | Methylorubrum | ACTTCATGGCCTTGCTCGG | GCGTGGTAGTGCAGGTTCT | 60.38 | 60.00 | 214 | 109 |
100.00%
|
50.00%
|
| Primer 98 | Methylorubrum | GCACTTCATGGCCTTGCTC | GCGTGGTAGTGCAGGTTCT | 59.49 | 60.00 | 216 | 109 |
100.00%
|
50.00%
|
| Primer 99 | Methylorubrum | TGGCAGAGAAGGGCAGTTC | ATCACGTCCTCGATGCTGG | 59.62 | 59.56 | 259 | 109 |
100.00%
|
25.00%
|
| Primer 100 | Methylorubrum | ACGCCAACGGTGACATCT | TCGCCTCGGTCACCTTTTC | 59.26 | 60.01 | 261 | 109 |
100.00%
|
50.00%
|