| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 71 | Methylorubrum | AACGTCCTCAAGGGCAAGG | GGAGCTCTTGATCACCGCA | 59.93 | 59.78 | 168 | 111 |
100.00%
|
50.00%
|
| Primer 72 | Methylorubrum | AAAGCCCTCTCGATCGCAG | AGTTGTCGCTGGAGAAGGC | 59.86 | 60.00 | 195 | 110 |
100.00%
|
50.00%
|
| Primer 73 | Methylorubrum | AAGCTCTCGAACGTCCTGC | TGACGTAGGTGCGGATCCA | 60.08 | 60.68 | 201 | 110 |
100.00%
|
50.00%
|
| Primer 74 | Methylorubrum | CGATCTCGGTGCTGATGCT | TGCCGTTGCCGAAGAACA | 59.93 | 60.20 | 237 | 110 |
100.00%
|
75.00%
|
| Primer 75 | Methylorubrum | TCGAAGCTCTCTCCTCGCT | ACATCACCGCTTCGACCA | 60.08 | 58.94 | 264 | 110 |
100.00%
|
25.00%
|
| Primer 76 | Methylorubrum | TCGAAGCTCTCTCCTCGCT | CATCACCGCTTCGACCACT | 60.08 | 60.08 | 263 | 110 |
100.00%
|
25.00%
|
| Primer 77 | Methylorubrum | TCGAAGCTCTCTCCTCGCT | GACATCACCGCTTCGACCA | 60.08 | 60.08 | 265 | 110 |
100.00%
|
25.00%
|
| Primer 78 | Methylorubrum | TCGAAGCTCTCTCCTCGCT | TCACCGCTTCGACCACTTG | 60.08 | 60.30 | 261 | 110 |
100.00%
|
25.00%
|
| Primer 79 | Methylorubrum | TCGAAGCTCTCTCCTCGCT | ACATCACCGCTTCGACCAC | 60.08 | 60.37 | 264 | 110 |
100.00%
|
25.00%
|
| Primer 80 | Methylorubrum | TCGAAGCTCTCTCCTCGCT | ATCACCGCTTCGACCACT | 60.08 | 58.62 | 262 | 110 |
100.00%
|
25.00%
|