| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Mycoplasmopsis | GCAATGATGGCCAAATGTGC | GGGAGCCAAACATTCGCTTG | 58.99 | 60.11 | 237 | 131 |
100.00%
|
40.00%
|
| Primer 2 | Mycoplasmopsis | GCTGTTGAGCCAAAGCCA | ACCTTGATTCTGTCGCCATCT | 58.55 | 59.44 | 206 | 127 |
100.00%
|
40.00%
|
| Primer 3 | Mycoplasmopsis | TGCTGTTGAGCCAAAGCC | ACCTTGATTCTGTCGCCATCT | 58.55 | 59.44 | 207 | 127 |
100.00%
|
40.00%
|
| Primer 4 | Mycoplasmopsis | TGCTGTTGAGCCAAAGCCA | ACCTTGATTCTGTCGCCATCT | 60.46 | 59.44 | 207 | 127 |
100.00%
|
40.00%
|
| Primer 5 | Mycoplasmopsis | GAGCCAAAGCCAATTGCCA | ACCTTGATTCTGTCGCCATCT | 59.33 | 59.44 | 200 | 127 |
100.00%
|
40.00%
|
| Primer 6 | Mycoplasmopsis | TGAGCCAAAGCCAATTGCC | ACCTTGATTCTGTCGCCATCT | 59.33 | 59.44 | 201 | 127 |
100.00%
|
40.00%
|
| Primer 7 | Mycoplasmopsis | AGCCAAAGCCAATTGCCAG | ACCTTGATTCTGTCGCCATCT | 59.32 | 59.44 | 199 | 127 |
100.00%
|
40.00%
|
| Primer 8 | Mycoplasmopsis | GCTGTTGAGCCAAAGCCAA | ACCTTGATTCTGTCGCCATCT | 59.26 | 59.44 | 206 | 127 |
100.00%
|
40.00%
|
| Primer 9 | Mycoplasmopsis | TTGCTGTTGAGCCAAAGCC | ACCTTGATTCTGTCGCCATCT | 59.26 | 59.44 | 208 | 127 |
100.00%
|
40.00%
|
| Primer 10 | Mycoplasmopsis | AGCCAAAGCCAATTGCCA | ACCTTGATTCTGTCGCCATCT | 58.11 | 59.44 | 199 | 127 |
100.00%
|
40.00%
|