| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Pyramidobacter | TTGCCCATCAAGGACACCG | ATTGGCGTTCGCAGAGCT | 60.30 | 60.05 | 271 | 81 |
100.00%
|
50.00%
|
| Primer 32 | Pyramidobacter | CCATCAAAGGCTCCTCGCT | TCTTCGCAGCGCTTTTGC | 59.78 | 59.75 | 169 | 81 |
100.00%
|
50.00%
|
| Primer 33 | Pyramidobacter | GTTCCGACATCTGCGTGGA | ATTTTCGCTCCGCTGGCT | 60.08 | 60.05 | 230 | 81 |
100.00%
|
50.00%
|
| Primer 34 | Pyramidobacter | TTCCGACATCTGCGTGGAG | ATTTTCGCTCCGCTGGCT | 59.78 | 60.05 | 229 | 81 |
100.00%
|
50.00%
|
| Primer 35 | Pyramidobacter | TGCTCGTGCTTTTCGGCT | GCGATCACGTAGCCGACAA | 60.28 | 60.52 | 223 | 81 |
100.00%
|
50.00%
|
| Primer 36 | Pyramidobacter | ATCCGCTCGTGATCAAGGC | TTTCAGCTCGACGACGCT | 60.23 | 59.36 | 167 | 81 |
100.00%
|
50.00%
|
| Primer 37 | Pyramidobacter | ACTGGTCATGCTGCTTGC | CAGCGGATGCCATTCTTGC | 58.32 | 59.93 | 163 | 80 |
100.00%
|
50.00%
|
| Primer 38 | Pyramidobacter | ATTCCGAACTCCACGCCA | AGCACGGCTTCGCTTTCT | 58.93 | 59.97 | 163 | 80 |
100.00%
|
50.00%
|
| Primer 39 | Pyramidobacter | TGGCAAACGACCTGGACT | AGCACGGCTTCGCTTTCT | 58.76 | 59.97 | 292 | 80 |
100.00%
|
50.00%
|
| Primer 40 | Pyramidobacter | GCAAACGACCTGGACTTGC | AGCACGGCTTCGCTTTCT | 59.72 | 59.97 | 290 | 80 |
100.00%
|
50.00%
|