| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Pyramidobacter | AAAGTGACGCTGCGCATC | TTCCATGCCCTGCTCCATC | 59.14 | 59.77 | 231 | 86 |
100.00%
|
50.00%
|
| Primer 12 | Pyramidobacter | AATACGCGTTGCTGCTGC | GCTTGAAAGCCGTTCGTGG | 59.52 | 60.08 | 239 | 86 |
100.00%
|
50.00%
|
| Primer 13 | Pyramidobacter | TGTGCGTGGTGATCTGCA | GTAAAACCACGGCTTCGCC | 59.57 | 59.79 | 212 | 85 |
100.00%
|
50.00%
|
| Primer 14 | Pyramidobacter | TGTGCGTGGTGATCTGCA | TAAAACCACGGCTTCGCC | 59.57 | 58.34 | 211 | 85 |
100.00%
|
50.00%
|
| Primer 15 | Pyramidobacter | TCGTCGTGTCGCGTTTCA | GCCAGTTCCTTCACGGTCA | 59.98 | 59.93 | 151 | 85 |
100.00%
|
50.00%
|
| Primer 16 | Pyramidobacter | ACCGAAGAAGAAGCCCAGC | TTCGTCTTGGTGCGCTGA | 60.00 | 59.58 | 173 | 85 |
100.00%
|
50.00%
|
| Primer 17 | Pyramidobacter | ATGGCATCGGTCTGAGCAC | TTCGTCTTGGTGCGCTGA | 60.15 | 59.58 | 250 | 85 |
100.00%
|
50.00%
|
| Primer 18 | Pyramidobacter | TGCTGTCTGGAAAGCGGA | ACACGTTGCCTTCGAGCA | 58.85 | 59.89 | 245 | 85 |
100.00%
|
50.00%
|
| Primer 19 | Pyramidobacter | TGCTCAGGGGATTGCTGTC | ACACGTTGCCTTCGAGCA | 59.70 | 59.89 | 257 | 85 |
100.00%
|
50.00%
|
| Primer 20 | Pyramidobacter | TGCTGTCTGGAAAGCGGAC | ACACGTTGCCTTCGAGCA | 60.30 | 59.89 | 245 | 85 |
100.00%
|
50.00%
|