| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Pyramidobacter | TACCGTTCGCGAAGGAAGG | ATGAAAACGCTGCGCACC | 59.79 | 59.74 | 289 | 117 |
100.00%
|
50.00%
|
| Primer 2 | Pyramidobacter | GGGGTACGAACGAAAACACC | AAACCAAGCGCAACGAGG | 59.13 | 58.97 | 183 | 106 |
100.00%
|
50.00%
|
| Primer 3 | Pyramidobacter | AAGCGCAGTACGTGGACTC | ATGAAAACGCTGCGCACC | 60.08 | 59.74 | 196 | 95 |
100.00%
|
50.00%
|
| Primer 4 | Pyramidobacter | TGTCCCGATCACGCAGTTC | AAGGTCGGCATCGCTGAT | 60.08 | 59.10 | 262 | 94 |
100.00%
|
50.00%
|
| Primer 5 | Pyramidobacter | ACAGCCTTGTCCCGATCAC | AAGGTCGGCATCGCTGAT | 59.70 | 59.10 | 269 | 94 |
100.00%
|
50.00%
|
| Primer 6 | Pyramidobacter | ACGCAGCAAAACGCAGTC | ACGGGATGAACCACGTAGC | 59.67 | 59.78 | 225 | 93 |
100.00%
|
50.00%
|
| Primer 7 | Pyramidobacter | TGAGCGAAGTTCACGGCA | AACGACAGGCAGCGCAT | 59.58 | 60.01 | 151 | 91 |
100.00%
|
50.00%
|
| Primer 8 | Pyramidobacter | ACAGTCGTCGTTTCGAGCA | TCGCCTTGAGCACGATGT | 59.64 | 59.34 | 192 | 91 |
100.00%
|
50.00%
|
| Primer 9 | Pyramidobacter | ACAACCTGATCGACGCCA | TCTTCGCAGCGCTTTTGC | 58.94 | 59.75 | 184 | 88 |
100.00%
|
50.00%
|
| Primer 10 | Pyramidobacter | AGCGATCGTCTTCACGGTG | TGGTGATGCAGCCGTACAC | 60.15 | 60.38 | 209 | 87 |
100.00%
|
50.00%
|