| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1261 | Ralstonia | TCCGAACACGCCTGCTTT | TGCCGAAATCCCAGCTGT | 59.89 | 59.24 | 286 | 53 |
100.00%
|
16.67%
|
| Primer 1262 | Ralstonia | ACGCCATCGACTTCGGTTT | ATTCACTCGTGCGTCGCT | 60.01 | 59.74 | 204 | 53 |
100.00%
|
16.67%
|
| Primer 1263 | Ralstonia | TCCTGGCAATTGTCGCGA | TCGCCAGCAACAACAGCA | 59.65 | 60.52 | 231 | 53 |
100.00%
|
16.67%
|
| Primer 1264 | Ralstonia | CTTGTCCTGTTTGCGCACG | ATGCTCTGCGTGCCGTTT | 60.37 | 60.67 | 179 | 53 |
100.00%
|
16.67%
|
| Primer 1265 | Ralstonia | TCTCCAAACGCATCGGCT | ACGTGCGGATCGTTTCGA | 59.34 | 59.74 | 273 | 53 |
100.00%
|
16.67%
|
| Primer 1266 | Ralstonia | TTCGTTGCCGTCGCTGAA | ACAGCGTTGATGCCACGA | 60.28 | 59.97 | 280 | 53 |
100.00%
|
16.67%
|
| Primer 1267 | Ralstonia | TGCTGCTGGTGGCCATTT | TGGGAACAGCACGCCAAA | 60.20 | 60.12 | 194 | 53 |
100.00%
|
16.67%
|
| Primer 1268 | Ralstonia | TGCGCAAGGTGTTGTCGA | AGCTCTTGAACGCGTCGA | 60.20 | 59.36 | 192 | 53 |
100.00%
|
16.67%
|
| Primer 1269 | Ralstonia | TGCATGGGTTGGTGGCAT | TCACGCGAAGGCTGCTTT | 59.88 | 60.28 | 244 | 53 |
100.00%
|
16.67%
|
| Primer 1270 | Ralstonia | TGGCCAACGCATGTACGA | CCTTCGCTTCCTCGTCGTT | 59.66 | 60.08 | 227 | 53 |
100.00%
|
33.33%
|