| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1271 | Ralstonia | TGGCCAACGCATGTACGA | CCTCGTCGTTCTTCGCAGT | 59.66 | 60.08 | 218 | 53 |
100.00%
|
33.33%
|
| Primer 1272 | Ralstonia | TGGCCAACGCATGTACGA | CGGGAACTTGAACGCGTTG | 59.66 | 60.08 | 253 | 53 |
100.00%
|
16.67%
|
| Primer 1273 | Ralstonia | TGGCCTGCGCTTTTACGA | GGCATCAATGGTGGCAAGC | 59.97 | 60.15 | 232 | 53 |
100.00%
|
16.67%
|
| Primer 1274 | Ralstonia | TCGACATTCCGCTTGCGA | ATGGCGATCGTGCGTTCA | 59.74 | 60.13 | 244 | 53 |
100.00%
|
33.33%
|
| Primer 1275 | Ralstonia | TCGACATTCCGCTTGCGA | ATCGTGCGTTCACCGTCA | 59.74 | 59.66 | 238 | 53 |
100.00%
|
33.33%
|
| Primer 1276 | Ralstonia | TCGACATTCCGCTTGCGA | TGTTCGGGAACTGCGTGT | 59.74 | 59.50 | 192 | 53 |
100.00%
|
16.67%
|
| Primer 1277 | Ralstonia | AGTCAGCATTGCCAGCGA | ACGACATTGCCGAACGGA | 59.65 | 59.66 | 183 | 53 |
100.00%
|
16.67%
|
| Primer 1278 | Ralstonia | TCGTCACACGCTCGTTGT | CCGACGAAGATCATGCCGA | 59.59 | 59.93 | 235 | 53 |
100.00%
|
16.67%
|
| Primer 1279 | Ralstonia | ATACGTGTGGTGTGCCGA | ACTGTGTGATGCCCTGAGC | 58.95 | 60.00 | 188 | 53 |
100.00%
|
16.67%
|
| Primer 1280 | Ralstonia | ATACGTGTGGTGTGCCGA | GTGTTCGCCTCGTACACCA | 58.95 | 60.01 | 216 | 53 |
100.00%
|
16.67%
|