| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 441 | Ralstonia | TCGAAGACGGGATTGCCTG | ACTGCCACTGCGAGCAAT | 59.78 | 59.97 | 252 | 86 |
100.00%
|
16.67%
|
| Primer 442 | Ralstonia | TCGGCAGCTTTGTCGTGT | CCCAGTTGAGCACGTCGAT | 59.89 | 60.08 | 183 | 85 |
100.00%
|
16.67%
|
| Primer 443 | Ralstonia | TGGTTTGGGTGTCGCGAT | TTTGCGTGACGTTGTGGC | 59.57 | 59.59 | 198 | 85 |
100.00%
|
16.67%
|
| Primer 444 | Ralstonia | TGACCAACTGACCGACGAC | AGCGCACGTCGACTTCAT | 59.64 | 59.74 | 191 | 85 |
100.00%
|
16.67%
|
| Primer 445 | Ralstonia | GGCCAATCGCAATGTCGAC | TTCACGCTCGTTGCCGAT | 59.94 | 60.05 | 292 | 85 |
100.00%
|
16.67%
|
| Primer 446 | Ralstonia | GCGTGCCGATCGTCAATTG | ATCCCACGCAATCCAGGAC | 60.30 | 59.78 | 159 | 85 |
100.00%
|
16.67%
|
| Primer 447 | Ralstonia | TACGTGTGGTGTGCCGAT | ACTGTGTGATGCCCTGAGC | 58.95 | 60.00 | 187 | 85 |
100.00%
|
16.67%
|
| Primer 448 | Ralstonia | ACATGTCGCTGGCCATGT | TCAGTTCCAGCACGAAGGC | 59.65 | 60.30 | 267 | 85 |
100.00%
|
33.33%
|
| Primer 449 | Ralstonia | CGGATCGACGTGATGGTGT | ACTTCAACCCCTCGCGAGT | 59.86 | 61.21 | 298 | 85 |
100.00%
|
16.67%
|
| Primer 450 | Ralstonia | AAGACTTCCCGAGCATGGC | ATGTCCATGCGACGCCAT | 60.08 | 59.81 | 236 | 85 |
100.00%
|
16.67%
|