| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 451 | Ralstonia | ACGTAACGGTGCAGCACA | ACGTTTCGTCAGTGCGCT | 59.89 | 60.28 | 165 | 84 |
100.00%
|
16.67%
|
| Primer 452 | Ralstonia | TCGCTTGGCCAAACTGCT | TTCGCGTGCAAACAGGTTG | 60.20 | 59.94 | 151 | 84 |
100.00%
|
33.33%
|
| Primer 453 | Ralstonia | TTCTGCGTGCTGGGTGAA | TGCGATAGTCGTACACGCC | 59.49 | 59.94 | 250 | 84 |
100.00%
|
16.67%
|
| Primer 454 | Ralstonia | TGCGATGCCATGAAGGGT | AGTTGGCGTCGTTGGTGT | 59.32 | 59.81 | 169 | 84 |
100.00%
|
16.67%
|
| Primer 455 | Ralstonia | TGCGGACCTGATTCCCAAC | GCCTTCCAGTTGCGATTGC | 60.00 | 60.15 | 271 | 84 |
100.00%
|
16.67%
|
| Primer 456 | Ralstonia | TTTCGGAATGGCAGGGGT | CAGACCATGAGCTGCACCA | 58.82 | 60.00 | 220 | 84 |
100.00%
|
16.67%
|
| Primer 457 | Ralstonia | TTCGGAGTTCGCCAAGCT | TGTCCATGCGTTGCGTCA | 59.26 | 60.28 | 182 | 84 |
100.00%
|
16.67%
|
| Primer 458 | Ralstonia | TCAAGCCTGAGCAACGCT | CTTCCTGTTGCCATCCGGT | 59.57 | 60.00 | 174 | 84 |
100.00%
|
16.67%
|
| Primer 459 | Ralstonia | TCAAGCCTGAGCAACGCT | GCCAATCACGAACGCGAT | 59.57 | 58.91 | 200 | 84 |
100.00%
|
16.67%
|
| Primer 460 | Ralstonia | TCAAGCCTGAGCAACGCT | CCATCCGGTGAGCGATTCA | 59.57 | 59.86 | 164 | 84 |
100.00%
|
16.67%
|