| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 501 | Rothia | ACGAAACCACCAAGCCCA | AGCAGGATCTGACCGGAGT | 59.40 | 60.00 | 157 | 68 |
100.00%
|
27.27%
|
| Primer 502 | Rothia | ACGAAACCACCAAGCCCA | ACAGCGGTCTTAGCGGAAC | 59.40 | 60.08 | 289 | 68 |
100.00%
|
27.27%
|
| Primer 503 | Rothia | AGCCGTCAACGAACAGCT | TGAGCTTGCGTGCTTCGT | 59.58 | 60.28 | 161 | 68 |
100.00%
|
27.27%
|
| Primer 504 | Rothia | TTCAAGAACCGCACCGCT | GCCTACTTCACCGAAGCGT | 59.89 | 60.08 | 204 | 68 |
100.00%
|
36.36%
|
| Primer 505 | Rothia | ATTTCACGGTGCTCGCGT | ATTCACCGCAGACGCCAA | 60.36 | 59.97 | 257 | 68 |
100.00%
|
18.18%
|
| Primer 506 | Rothia | TGCCAGCACTTTACGCCT | ATCTTTGGCGACCCAGAGG | 59.57 | 59.40 | 176 | 67 |
100.00%
|
18.18%
|
| Primer 507 | Rothia | TACGCAGAGAACGTCGCA | TGCGCTGTGAACGGGTTA | 59.05 | 59.58 | 181 | 67 |
100.00%
|
27.27%
|
| Primer 508 | Rothia | TGTTCCTGCGTGCGTCTA | TGCGCTGTGAACGGGTTA | 58.96 | 59.58 | 210 | 67 |
100.00%
|
27.27%
|
| Primer 509 | Rothia | CTGCGTGCGTCTATTTGGC | TGCGCTGTGAACGGGTTA | 59.94 | 59.58 | 205 | 67 |
100.00%
|
27.27%
|
| Primer 510 | Rothia | CTGTTCCTGCGTGCGTCTA | TGCGCTGTGAACGGGTTA | 60.08 | 59.58 | 211 | 67 |
100.00%
|
27.27%
|