| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 521 | Rothia | AAGGGCTTCGCAGGTGTT | TGCACCCTTGATCAGCAGG | 59.48 | 60.00 | 231 | 66 |
100.00%
|
45.45%
|
| Primer 522 | Rothia | AGCTATCACCGAGCGCAA | ATGCGTCGAATGCTGCCT | 59.11 | 60.12 | 173 | 66 |
100.00%
|
36.36%
|
| Primer 523 | Rothia | TCACCGAGCGCAAGAACAT | ATGCGTCGAATGCTGCCT | 60.01 | 60.12 | 168 | 66 |
100.00%
|
36.36%
|
| Primer 524 | Rothia | ATCACCGAGCGCAAGAACA | ATGCGTCGAATGCTGCCT | 60.01 | 60.12 | 169 | 66 |
100.00%
|
36.36%
|
| Primer 525 | Rothia | TGCGTGCGAACATCGACT | TGCCGAAGCGTTCTCGTT | 60.05 | 59.97 | 215 | 66 |
100.00%
|
45.45%
|
| Primer 526 | Rothia | TCGGCACCGTGATTGGTT | TTCTTCGGCAGCAGCGAA | 59.57 | 59.97 | 175 | 66 |
100.00%
|
18.18%
|
| Primer 527 | Rothia | ACTGGAGCTTCCGCAGTTC | GCAATCTCGGTGAAGCCCT | 60.00 | 60.08 | 192 | 66 |
100.00%
|
27.27%
|
| Primer 528 | Rothia | GCACCTGTTTGCGCAGTT | TGCAGGCCAGAACACGTT | 59.59 | 59.81 | 181 | 66 |
100.00%
|
36.36%
|
| Primer 529 | Rothia | TCTGCACCTGTTTGCGCA | TGCAGGCCAGAACACGTT | 60.52 | 59.81 | 184 | 66 |
100.00%
|
36.36%
|
| Primer 530 | Rothia | TTCTGCACCTGTTTGCGC | TGCAGGCCAGAACACGTT | 59.28 | 59.81 | 185 | 66 |
100.00%
|
36.36%
|