| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 531 | Rothia | TTCGCAGCGCTGGAAGAA | GCGAGGCTTTCAGTGTCCT | 59.97 | 60.00 | 152 | 66 |
100.00%
|
27.27%
|
| Primer 532 | Rothia | GACCTTCCGGTGGCAATCA | ACCCACGCAACATCGACT | 60.00 | 59.26 | 296 | 65 |
100.00%
|
27.27%
|
| Primer 533 | Rothia | TCGCAGCAATTGAGCCGA | CAATAATGCGTGCGGAGGC | 60.05 | 60.01 | 189 | 65 |
100.00%
|
18.18%
|
| Primer 534 | Rothia | TCGCAGCAATTGAGCCGA | GCAATAATGCGTGCGGAGG | 60.05 | 60.01 | 190 | 65 |
100.00%
|
18.18%
|
| Primer 535 | Rothia | TCGCAGCAATTGAGCCGA | AGGATACCAACGGTGCAGC | 60.05 | 60.08 | 159 | 65 |
100.00%
|
18.18%
|
| Primer 536 | Rothia | TGTTTGCCCTGATGCCGA | AATCCTCTGCGTGTGCCA | 59.57 | 59.25 | 231 | 65 |
100.00%
|
18.18%
|
| Primer 537 | Rothia | TCGCGAATACGATGCCGA | ATGCGCTTGTCCATCGGT | 59.28 | 59.73 | 213 | 65 |
100.00%
|
18.18%
|
| Primer 538 | Rothia | GCCGTACTCGCGAATACGA | ATGCGCTTGTCCATCGGT | 60.01 | 59.73 | 220 | 65 |
100.00%
|
18.18%
|
| Primer 539 | Rothia | AGCGCATGCACATGGAGT | GTTTCGCATCATCGGTGGC | 60.05 | 59.94 | 153 | 65 |
100.00%
|
18.18%
|
| Primer 540 | Rothia | ACCGCAAAGTCGTGCTGA | TTTGTCGCTGCGGTTGTG | 59.89 | 59.29 | 260 | 65 |
100.00%
|
18.18%
|