| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Thermotoga | TTGGAGTGACTGCTGCTGC | TGACTGCATCGGGTGAACC | 60.60 | 60.00 | 219 | 168 |
100.00%
|
80.00%
|
| Primer 2 | Thermotoga | ACTGGCCACGGTGATTCTG | TCGTCAGCAGTCCAAGAGC | 60.00 | 59.71 | 152 | 165 |
100.00%
|
100.00%
|
| Primer 3 | Thermotoga | TCTTGCCGTGAAGCTCACC | CGATGAGAGACCTGGCCTTC | 60.30 | 59.90 | 158 | 151 |
100.00%
|
80.00%
|
| Primer 4 | Thermotoga | TGGTGTTGCTCGGCATGT | GTCCGTCCGTCAGGTTCAT | 59.89 | 59.41 | 264 | 148 |
100.00%
|
60.00%
|
| Primer 5 | Thermotoga | TGGTGTTGCTCGGCATGT | TGGCGTTCGAACTTCCCAC | 59.89 | 60.60 | 246 | 148 |
100.00%
|
60.00%
|
| Primer 6 | Thermotoga | ACTGCTGCTGCACCTGT | TGACTGCATCGGGTGAACC | 59.08 | 60.00 | 211 | 148 |
100.00%
|
80.00%
|
| Primer 7 | Thermotoga | TGTGCAAGGGGAAGTACCAG | ACGTTGACGATGAAAGGAAGG | 59.60 | 58.59 | 203 | 148 |
100.00%
|
40.00%
|
| Primer 8 | Thermotoga | ACAACGTGTCCGCACTGT | CGACAAAGCCATTCTGCTGC | 59.81 | 60.46 | 157 | 148 |
100.00%
|
80.00%
|
| Primer 9 | Thermotoga | ACAACGTGTCCGCACTGT | AGCCATTCTGCTGCAACTC | 59.81 | 58.44 | 151 | 148 |
100.00%
|
80.00%
|
| Primer 10 | Thermotoga | TGTGCAAGGGGAAGTACCAG | ACGTTGACGATGAAAGGAAGG | 59.60 | 58.59 | 203 | 148 |
100.00%
|
40.00%
|