| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Thermotoga | AGGTCGCAACGTGGTGAT | CCTCAGTCACGGCAGATGT | 59.26 | 59.41 | 170 | 141 |
100.00%
|
80.00%
|
| Primer 32 | Thermotoga | TCTCGAGCGAGAACCCGAT | AGGGGATTGGGGAGGAGAT | 60.45 | 58.95 | 261 | 141 |
100.00%
|
40.00%
|
| Primer 33 | Thermotoga | ACAACGTGTCCGCACTGT | TGCTTTGAACACCTCCGGA | 59.81 | 59.17 | 183 | 140 |
100.00%
|
80.00%
|
| Primer 34 | Thermotoga | ACAACGTGTCCGCACTGT | CTGCTTTGAACACCTCCGGA | 59.81 | 60.25 | 184 | 140 |
100.00%
|
80.00%
|
| Primer 35 | Thermotoga | AACTGGAGTGCGGAAGGTG | AAGTGGCTCGCCTCTTTCC | 59.93 | 60.00 | 254 | 140 |
100.00%
|
100.00%
|
| Primer 36 | Thermotoga | AACTGGAGTGCGGAAGGTG | AAGTGGCTCGCCTCTTTCC | 59.93 | 60.00 | 254 | 140 |
100.00%
|
100.00%
|
| Primer 37 | Thermotoga | AACTGGAGTGCGGAAGGTG | AAGTGGCTCGCCTCTTTCC | 59.93 | 60.00 | 254 | 140 |
100.00%
|
100.00%
|
| Primer 38 | Thermotoga | TTACGGCTTGCTCACGCA | TCGCTGTGAGAACGAGAGC | 59.97 | 59.79 | 191 | 138 |
100.00%
|
40.00%
|
| Primer 39 | Thermotoga | TTACGGCTTGCTCACGCA | AAGAGAAGCTCCGAGTGCC | 59.97 | 59.41 | 232 | 138 |
100.00%
|
40.00%
|
| Primer 40 | Thermotoga | TCTTGCCGTGAAGCTCACC | TCAGCGATGAGAGACCTGG | 60.30 | 58.50 | 162 | 138 |
100.00%
|
80.00%
|