| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Thermotoga | TGCTGCCTTTGACGCACT | AAAGCCCATCAGCGAGGA | 60.20 | 58.60 | 203 | 138 |
100.00%
|
60.00%
|
| Primer 52 | Thermotoga | TGCTGCCTTTGACGCACT | TGACTCTGCAACTTCCGCA | 60.20 | 59.55 | 280 | 138 |
100.00%
|
60.00%
|
| Primer 53 | Thermotoga | TCGTGGCTGAAGAGCTTGG | ACCATGATCACCGGTGCA | 60.00 | 58.92 | 265 | 138 |
100.00%
|
80.00%
|
| Primer 54 | Thermotoga | TCGTGGCTGAAGAGCTTGG | TGCAACACCACAAAGGCC | 60.00 | 58.78 | 251 | 138 |
100.00%
|
80.00%
|
| Primer 55 | Thermotoga | TCGTGGCTGAAGAGCTTGG | GTGCAACACCACAAAGGCC | 60.00 | 60.23 | 252 | 138 |
100.00%
|
80.00%
|
| Primer 56 | Thermotoga | TCGTGGCTGAAGAGCTTGG | CACCATGATCACCGGTGCA | 60.00 | 60.38 | 266 | 138 |
100.00%
|
80.00%
|
| Primer 57 | Thermotoga | ACAACGTGTCCGCACTGT | CAAAGCCGTTCTGCTGCA | 59.81 | 58.97 | 154 | 138 |
100.00%
|
80.00%
|
| Primer 58 | Thermotoga | TCTCATCACACTCGCGTGG | ACGCCAAGAACCACCTTCC | 59.79 | 60.23 | 225 | 138 |
100.00%
|
80.00%
|
| Primer 59 | Thermotoga | AACACCCCTGTGCCAGATG | TGCAGTCCGGTTTGCACA | 59.92 | 60.12 | 225 | 137 |
100.00%
|
80.00%
|
| Primer 60 | Thermotoga | GAACACCCCTGTGCCAGAT | TGCAGTCCGGTTTGCACA | 59.62 | 60.12 | 226 | 137 |
100.00%
|
80.00%
|