| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Thermotoga | TCTTGCCGTGAAGCTCACC | TGGCAGTCTCGAACAGATCC | 60.30 | 59.47 | 188 | 138 |
100.00%
|
80.00%
|
| Primer 42 | Thermotoga | AGCCATCGCACATGCACT | CTTCCACCTTTCCACCGGA | 60.05 | 59.24 | 265 | 138 |
100.00%
|
40.00%
|
| Primer 43 | Thermotoga | AGCCATCGCACATGCACT | TTCCACCTTTCCACCGGA | 60.05 | 58.01 | 264 | 138 |
100.00%
|
40.00%
|
| Primer 44 | Thermotoga | AGCCATCGCACATGCACT | ACCTTCTTCACCGCATGGA | 60.05 | 58.93 | 171 | 138 |
100.00%
|
40.00%
|
| Primer 45 | Thermotoga | AGCCATCGCACATGCACT | CCACCGGATAGTTCTCTGCC | 60.05 | 59.89 | 254 | 138 |
100.00%
|
40.00%
|
| Primer 46 | Thermotoga | CACCCCATTGGCCTCACAT | CCAAGGAGAAGCCCAAGCA | 60.00 | 59.92 | 292 | 138 |
100.00%
|
60.00%
|
| Primer 47 | Thermotoga | TGCTGCCTTTGACGCACT | AAGCCCATCAGCGAGGAAC | 60.20 | 60.08 | 202 | 138 |
100.00%
|
60.00%
|
| Primer 48 | Thermotoga | TGCTGCCTTTGACGCACT | GAAAGCCCATCAGCGAGGA | 60.20 | 59.78 | 204 | 138 |
100.00%
|
60.00%
|
| Primer 49 | Thermotoga | TGCTGCCTTTGACGCACT | AGAAAGCCCATCAGCGAGG | 60.20 | 59.78 | 205 | 138 |
100.00%
|
60.00%
|
| Primer 50 | Thermotoga | TGCTGCCTTTGACGCACT | AGGAACGCCTGACCAGTTC | 60.20 | 59.63 | 189 | 138 |
100.00%
|
60.00%
|