| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1001 | Thermotoga | CAACCCCACAAGTTACCGGA | TCCAGCAGATCCAGCACA | 59.89 | 58.19 | 269 | 93 |
100.00%
|
80.00%
|
| Primer 1002 | Thermotoga | ACCCAAAGGATGCCGAACA | TCGCTGCGTTCGCTTCAT | 59.54 | 60.43 | 162 | 93 |
100.00%
|
60.00%
|
| Primer 1003 | Thermotoga | ACGTGGCAGCCGTGAAAA | ACGAACTGAGGTGTGAGGC | 60.52 | 59.63 | 232 | 93 |
100.00%
|
80.00%
|
| Primer 1004 | Thermotoga | GGGTTTTGGAACGTGCTGG | GTGAGGTACGCGTGTGACA | 59.64 | 60.01 | 297 | 93 |
100.00%
|
80.00%
|
| Primer 1005 | Thermotoga | CCAAAGTGTCCGGTCCCAT | TCTCAACGTCGACACCTGC | 59.62 | 60.01 | 198 | 93 |
100.00%
|
100.00%
|
| Primer 1006 | Thermotoga | TCTCATCACACTCGCGTGG | ACCACCTTCCATGCCACA | 59.79 | 58.74 | 216 | 93 |
100.00%
|
80.00%
|
| Primer 1007 | Thermotoga | GGACTCGTGAGGGCACAAA | TTTCCGGTTGTACGCTCCC | 59.93 | 60.00 | 171 | 93 |
100.00%
|
40.00%
|
| Primer 1008 | Thermotoga | ACGGTTCACTGTCGCACA | TCACGTCCACATCCGCAT | 59.50 | 59.02 | 198 | 93 |
100.00%
|
40.00%
|
| Primer 1009 | Thermotoga | ACGGTTCACTGTCGCACA | CTCACGTCCACATCCGCAT | 59.50 | 60.15 | 199 | 93 |
100.00%
|
40.00%
|
| Primer 1010 | Thermotoga | ACGGTTCACTGTCGCACA | TCACGTCCACATCCGCATC | 59.50 | 60.15 | 198 | 93 |
100.00%
|
40.00%
|