| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1011 | Thermotoga | ACAAGGTGGCGTGTCACA | ACCGCTTCTTCACCGAACT | 59.41 | 59.25 | 205 | 93 |
100.00%
|
40.00%
|
| Primer 1012 | Thermotoga | ACCTGGCGTCTTGTGAAGG | TCAGCGTGTGGGCTTCTT | 59.93 | 59.17 | 184 | 92 |
100.00%
|
80.00%
|
| Primer 1013 | Thermotoga | TCCGCTGTGAGACCTCAGA | TCAGCGTGTGGGCTTCTT | 59.93 | 59.17 | 208 | 92 |
100.00%
|
80.00%
|
| Primer 1014 | Thermotoga | CGCACCCCTTGGAAGTCTT | TTGCGTCCACGTTGACCA | 59.93 | 59.81 | 209 | 92 |
100.00%
|
40.00%
|
| Primer 1015 | Thermotoga | CTTGCCCTCTGCGGAAAGA | TCCAAGATGGCAGCTGCA | 60.00 | 59.24 | 254 | 92 |
100.00%
|
40.00%
|
| Primer 1016 | Thermotoga | TTGCCCTCTGCGGAAAGA | TCCAAGATGGCAGCTGCA | 58.84 | 59.24 | 253 | 92 |
100.00%
|
40.00%
|
| Primer 1017 | Thermotoga | TCGAGCCCATCACTCCAGA | CAGAGGGTTGTCACACGCT | 60.00 | 59.93 | 245 | 92 |
100.00%
|
80.00%
|
| Primer 1018 | Thermotoga | TCGAGCCCATCACTCCAGA | ACAGAGGGTTGTCACACGC | 60.00 | 60.23 | 246 | 92 |
100.00%
|
80.00%
|
| Primer 1019 | Thermotoga | GGCTGAACGGTTGACCCTT | TGGTGACAAGGAGCGCTAC | 60.23 | 59.71 | 254 | 92 |
100.00%
|
80.00%
|
| Primer 1020 | Thermotoga | TTTCCGGTGAACGTGGTGG | CGTTCATGTGACGCACCAG | 60.52 | 59.51 | 214 | 92 |
100.00%
|
60.00%
|