| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1391 | Thermotoga | GGTGTCCGAGGTGCAAACA | ACTTTTCCGTCCACCGCT | 60.53 | 59.17 | 151 | 84 |
100.00%
|
100.00%
|
| Primer 1392 | Thermotoga | CTGGCCTTCTGGAAGTGGT | ACGGTCTGTCAGCTCCTGT | 59.24 | 60.53 | 225 | 84 |
100.00%
|
40.00%
|
| Primer 1393 | Thermotoga | ACCCGATGGAGCCAAGAA | ACGGTCTGTCAGCTCCTGT | 58.18 | 60.53 | 272 | 84 |
100.00%
|
40.00%
|
| Primer 1394 | Thermotoga | ACCCGATGGAGCCAAGAAAG | ACGGTCTGTCAGCTCCTGT | 60.04 | 60.53 | 272 | 84 |
100.00%
|
40.00%
|
| Primer 1395 | Thermotoga | ACTGTTGCCCTCGCATCT | TTCTGAACGTCGTCCACCG | 58.93 | 60.01 | 296 | 83 |
100.00%
|
60.00%
|
| Primer 1396 | Thermotoga | CTGTTGCCCTCGCATCTCT | TTCTGAACGTCGTCCACCG | 59.78 | 60.01 | 295 | 83 |
100.00%
|
60.00%
|
| Primer 1397 | Thermotoga | TGGAGTACGCCAGGGAGAA | TTGCGTTCGCTCCCATTCT | 59.92 | 60.00 | 264 | 83 |
100.00%
|
80.00%
|
| Primer 1398 | Thermotoga | TCCCGTTGAGCTGCGAAA | CAACCGTTCAGCCACCTCT | 59.58 | 59.93 | 255 | 83 |
100.00%
|
40.00%
|
| Primer 1399 | Thermotoga | TTCCCGTTGAGCTGCGAA | CAACCGTTCAGCCACCTCT | 59.58 | 59.93 | 256 | 83 |
100.00%
|
40.00%
|
| Primer 1400 | Thermotoga | TGTTGTCGCAGCGCTTCT | ATTGCCTCAAGGAGCGCA | 60.28 | 59.65 | 285 | 83 |
100.00%
|
80.00%
|