| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1411 | Thermotoga | TGTTGTCGCAGCGCTTCT | GTTCTCGGTGTTGCCCTGT | 60.28 | 60.23 | 261 | 83 |
100.00%
|
80.00%
|
| Primer 1412 | Thermotoga | GGTGCCCATTCCCATCGAT | TCACATCACCGGTTGTGGG | 59.85 | 59.93 | 281 | 82 |
100.00%
|
40.00%
|
| Primer 1413 | Thermotoga | ACCAACCGAGGAATCGCA | TGTCGAGAAGGTTGCCGAG | 58.93 | 59.71 | 175 | 82 |
100.00%
|
60.00%
|
| Primer 1414 | Thermotoga | AGCCATCGCAACGATAGGG | TCGTGTTCCCGTTGCTGT | 59.93 | 59.50 | 230 | 82 |
100.00%
|
40.00%
|
| Primer 1415 | Thermotoga | TCACGACCACAAACGGCA | CGCCAGCGAGCAGAAAATC | 59.81 | 59.94 | 177 | 82 |
100.00%
|
80.00%
|
| Primer 1416 | Thermotoga | ACGACCACAAACGGCACT | CGCCAGCGAGCAGAAAATC | 59.81 | 59.94 | 175 | 82 |
100.00%
|
80.00%
|
| Primer 1417 | Thermotoga | ACATGCATCGGTTCTGGCC | TTGCAACCTCCACCGCAT | 60.75 | 59.88 | 186 | 82 |
100.00%
|
40.00%
|
| Primer 1418 | Thermotoga | AAAGTCGGAGCCATGGCA | ACTCCTTGCTCTGTTGCCC | 59.24 | 59.93 | 282 | 82 |
100.00%
|
80.00%
|
| Primer 1419 | Thermotoga | ATTGCATCGGACCACGCA | TCTCGCGAGTCTTGCCATG | 60.05 | 60.15 | 276 | 82 |
100.00%
|
80.00%
|
| Primer 1420 | Thermotoga | TGAACGATTTCGCCACGGA | GGATGTTCTCTGGCGAGCA | 60.01 | 59.78 | 163 | 82 |
100.00%
|
40.00%
|