| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1421 | Thermotoga | ACGGACTCCAGTGTTGCA | GGATGTTCTCTGGCGAGCA | 58.77 | 59.78 | 227 | 82 |
100.00%
|
40.00%
|
| Primer 1422 | Thermotoga | CGATCATTGTGGGAGTGGCA | GCTGGGCAAAGCAACATCC | 60.39 | 60.08 | 239 | 82 |
100.00%
|
60.00%
|
| Primer 1423 | Thermotoga | AAGAGGTTTCGGCAGAGGC | AAGCTGGCAGTACACGCA | 60.00 | 59.57 | 173 | 82 |
100.00%
|
40.00%
|
| Primer 1424 | Thermotoga | GAGGTTTCGGCAGAGGCAT | AAGCTGGCAGTACACGCA | 60.08 | 59.57 | 171 | 82 |
100.00%
|
40.00%
|
| Primer 1425 | Thermotoga | AGGTTTCGGCAGAGGCAT | AAGCTGGCAGTACACGCA | 58.92 | 59.57 | 170 | 82 |
100.00%
|
40.00%
|
| Primer 1426 | Thermotoga | TGGCAAGGGTAGCGATTCC | ACGCGTTTCTGCCGACAT | 59.78 | 60.36 | 225 | 82 |
100.00%
|
40.00%
|
| Primer 1427 | Thermotoga | GAAGATCATCGCCAGGGCA | ATGTGGCGAGCACAAGCA | 59.85 | 60.28 | 153 | 82 |
100.00%
|
40.00%
|
| Primer 1428 | Thermotoga | TGAAGATCATCGCCAGGGC | ATGTGGCGAGCACAAGCA | 59.85 | 60.28 | 154 | 82 |
100.00%
|
40.00%
|
| Primer 1429 | Thermotoga | AAGATCATCGCCAGGGCAG | ATGTGGCGAGCACAAGCA | 59.85 | 60.28 | 152 | 82 |
100.00%
|
40.00%
|
| Primer 1430 | Thermotoga | TGGATGGCTCAGTGGGACT | AACAGAATAGCCAGGTGCCC | 60.23 | 60.03 | 186 | 82 |
100.00%
|
40.00%
|