| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1471 | Thermotoga | TTGCCGTACTCTCTGGAGC | AAACGCGACGGTGGAAGA | 59.11 | 59.58 | 212 | 81 |
100.00%
|
60.00%
|
| Primer 1472 | Thermotoga | TGTGGTGGGTGGGGATCTT | TGTTTCCTGCGTGTGTGC | 60.47 | 58.89 | 151 | 81 |
100.00%
|
60.00%
|
| Primer 1473 | Thermotoga | TTCGAAGCGCTTCTGGCT | GGTGGCACTTTGTGTTCGC | 59.66 | 60.30 | 170 | 81 |
100.00%
|
40.00%
|
| Primer 1474 | Thermotoga | GAAAAGCCCACACCCGAGA | TCCACCTGCACCTCAAACC | 59.93 | 59.85 | 214 | 81 |
100.00%
|
80.00%
|
| Primer 1475 | Thermotoga | AGAAAAGCCCACACCCGAG | TCCACCTGCACCTCAAACC | 59.93 | 59.85 | 215 | 81 |
100.00%
|
80.00%
|
| Primer 1476 | Thermotoga | TCCAAGTACGAGGTTGGACA | TCACTGCTGCTGGGCAAA | 58.29 | 59.80 | 158 | 81 |
100.00%
|
60.00%
|
| Primer 1477 | Thermotoga | CAACGATCATTGTGGGAGTGG | TCACTGCTGCTGGGCAAA | 59.26 | 59.80 | 250 | 81 |
100.00%
|
60.00%
|
| Primer 1478 | Thermotoga | GTTGTTGTCGCAGCGCTT | ATTGCCTCAAGGAGCGCA | 59.67 | 59.65 | 287 | 81 |
100.00%
|
80.00%
|
| Primer 1479 | Thermotoga | AAGTTGTTGTCGCAGCGC | ATTGCCTCAAGGAGCGCA | 59.67 | 59.65 | 289 | 81 |
100.00%
|
80.00%
|
| Primer 1480 | Thermotoga | AGCGCTCGATGCTTCCTT | GTGGGGTCGTGGTCTTTCA | 59.42 | 59.55 | 195 | 81 |
100.00%
|
80.00%
|