| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1491 | Thermotoga | CCGACAGACGTGCTCACTT | CACCCCATACCTCCTCAACG | 60.01 | 59.82 | 238 | 81 |
100.00%
|
40.00%
|
| Primer 1492 | Thermotoga | CGTGAGATTGTCGCTGGAGA | TCGTCTGGCGACTTTGCA | 59.83 | 59.58 | 171 | 81 |
100.00%
|
80.00%
|
| Primer 1493 | Thermotoga | AGGGGTGTCGAGCTGAAGA | AACGGCTGCTGTGTCTGT | 60.23 | 59.49 | 261 | 81 |
100.00%
|
40.00%
|
| Primer 1494 | Thermotoga | TAGCCCACAGCCCAGAAGA | CTGAACCGTCGTGTCCAGT | 60.23 | 59.64 | 158 | 81 |
100.00%
|
80.00%
|
| Primer 1495 | Thermotoga | TAGCCCACAGCCCAGAAGA | TTCAGTCTGCCCTGAACCG | 60.23 | 59.63 | 169 | 81 |
100.00%
|
80.00%
|
| Primer 1496 | Thermotoga | CGATGGACGTCTCCGGAAA | ACAGCCACCCTTTCGTAGC | 59.49 | 60.00 | 274 | 81 |
100.00%
|
80.00%
|
| Primer 1497 | Thermotoga | ACGTGGCAGCCGTGAAAA | ACGAACTGAGGTGTGAGGC | 60.52 | 59.63 | 232 | 81 |
100.00%
|
80.00%
|
| Primer 1498 | Thermotoga | GCCATCGGAATCGCAGAGA | TCCGTCATTTGCTGCGGT | 59.93 | 59.97 | 171 | 81 |
100.00%
|
80.00%
|
| Primer 1499 | Thermotoga | TCCCGTGATGTTTGCCTGT | CCTTTCCAGCACTGCCAAC | 59.55 | 59.34 | 263 | 81 |
100.00%
|
100.00%
|
| Primer 1500 | Thermotoga | TCCAAGTACGAGGTTGGACA | TCACTGCTGCTGGGCAAA | 58.29 | 59.80 | 158 | 81 |
100.00%
|
60.00%
|