| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2211 | Thermotoga | AAGGCAGCGGTTGAGGTT | TCTTCGGGATGAGAAGCGG | 59.48 | 59.18 | 269 | 66 |
100.00%
|
60.00%
|
| Primer 2212 | Thermotoga | TGGGAACGGGTGTTGTCAG | GTGGTGACAAGGAGCGCTA | 59.85 | 59.71 | 160 | 66 |
100.00%
|
60.00%
|
| Primer 2213 | Thermotoga | GGAACGGGTGTTGTCAGCT | GTGGTGACAAGGAGCGCTA | 60.23 | 59.71 | 158 | 66 |
100.00%
|
60.00%
|
| Primer 2214 | Thermotoga | GAAAAGCCCACACCCGAGA | CCACCTGCACCTCAAACCT | 59.93 | 59.85 | 213 | 66 |
100.00%
|
80.00%
|
| Primer 2215 | Thermotoga | GGCTCGTGATCACCAGGTT | TGGGAAGGAACATCACCGC | 59.70 | 60.00 | 153 | 66 |
100.00%
|
40.00%
|
| Primer 2216 | Thermotoga | CACAGAAAGCACGGGACCT | ATCCACGCTGTCAAGGACG | 59.93 | 60.08 | 180 | 66 |
100.00%
|
80.00%
|
| Primer 2217 | Thermotoga | GCTGGTACCGCTTGCAAA | CGTGGAGTCGGCAAGAGTT | 58.65 | 60.01 | 276 | 66 |
100.00%
|
60.00%
|
| Primer 2218 | Thermotoga | ACGTGGAGCAGAGCCTCTA | GTCGGCATGACCTCCAAATC | 60.00 | 58.98 | 159 | 66 |
100.00%
|
80.00%
|
| Primer 2219 | Thermotoga | AAACGTGGAGCAGAGCCT | TCGGCATGACCTCCAAATCT | 58.85 | 59.09 | 160 | 66 |
100.00%
|
80.00%
|
| Primer 2220 | Thermotoga | TAAACGAAGCGCTCCGGA | CGAGCTCGTTCATTTCCCCT | 59.04 | 60.11 | 165 | 66 |
100.00%
|
80.00%
|