| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2221 | Thermotoga | AAGCCGTTCAGCGTGAGA | GCGAGGAATCGGAGCAGTT | 59.27 | 60.15 | 203 | 66 |
100.00%
|
60.00%
|
| Primer 2222 | Thermotoga | TTCCTGAGCGCAAAGGGAG | GCGAGGAATCGGAGCAGTT | 60.00 | 60.15 | 275 | 66 |
100.00%
|
60.00%
|
| Primer 2223 | Thermotoga | CTTCCTGAGCGCAAAGGGA | GCGAGGAATCGGAGCAGTT | 60.00 | 60.15 | 276 | 66 |
100.00%
|
60.00%
|
| Primer 2224 | Thermotoga | CAACTGGAGTGCGGAAGGT | GGTCTTCCACACCGGTGTT | 59.93 | 59.85 | 281 | 66 |
100.00%
|
100.00%
|
| Primer 2225 | Thermotoga | AACTGGAGTGCGGAAGGTG | CTGAGAAAGTGGCTCGCCT | 59.93 | 59.71 | 260 | 66 |
100.00%
|
100.00%
|
| Primer 2226 | Thermotoga | TCGCACTCGATGGCATCA | ATTCGGCACCGTTCGCTA | 59.11 | 59.43 | 288 | 66 |
100.00%
|
40.00%
|
| Primer 2227 | Thermotoga | TCTTCGCACTCGATGGCA | ATTCGGCACCGTTCGCTA | 59.03 | 59.43 | 291 | 66 |
100.00%
|
40.00%
|
| Primer 2228 | Thermotoga | TGCTCTTCGACGAACCCAC | CGAGAAACTCACGCGTCCT | 60.01 | 60.08 | 237 | 66 |
100.00%
|
80.00%
|
| Primer 2229 | Thermotoga | CAACTGGAGTGCGGAAGGT | GGTCTTCCACACCGGTGTT | 59.93 | 59.85 | 281 | 66 |
100.00%
|
100.00%
|
| Primer 2230 | Thermotoga | AACTGGAGTGCGGAAGGTG | CTGAGAAAGTGGCTCGCCT | 59.93 | 59.71 | 260 | 66 |
100.00%
|
100.00%
|