| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2321 | Thermotoga | AGCCGAACATCTCACAGCA | TCCTTTCTCGCACGTTCGA | 59.33 | 59.34 | 151 | 65 |
100.00%
|
80.00%
|
| Primer 2322 | Thermotoga | TCAAAGGGCACGATGCGA | TCGTTGCCTCTTCCTGGT | 59.65 | 58.11 | 243 | 65 |
100.00%
|
80.00%
|
| Primer 2323 | Thermotoga | TCAAAGGGCACGATGCGA | CTTCGTTGCCTCTTCCTGGT | 59.65 | 59.96 | 245 | 65 |
100.00%
|
100.00%
|
| Primer 2324 | Thermotoga | ATCCCGTACGGTGAGACGA | TGCGCTGAAACCACCGA | 60.08 | 59.52 | 156 | 65 |
100.00%
|
60.00%
|
| Primer 2325 | Thermotoga | ATCCCGTACGGTGAGACGA | AGCCTGCGCTGAAACCA | 60.08 | 59.51 | 160 | 65 |
100.00%
|
60.00%
|
| Primer 2326 | Thermotoga | ATCCCGTACGGTGAGACGA | CTTCCATTCCAAGCCTGCG | 60.08 | 59.19 | 171 | 65 |
100.00%
|
60.00%
|
| Primer 2327 | Thermotoga | TGCGCAACCTGTGATGGA | AGAACTTTCGCAGCCGTGA | 59.57 | 59.93 | 155 | 65 |
100.00%
|
40.00%
|
| Primer 2328 | Thermotoga | TCGTACTGCGCAACCTGT | AGAACTTTCGCAGCCGTGA | 59.27 | 59.93 | 161 | 65 |
100.00%
|
80.00%
|
| Primer 2329 | Thermotoga | AGAGTTGTGGGCTGTCACG | TGGCGAGCTTCACGTTCA | 59.93 | 59.58 | 166 | 65 |
100.00%
|
80.00%
|
| Primer 2330 | Thermotoga | GGGGACCGAATTCTCGTGA | CCAAAGCTTTCCACCGTGC | 59.10 | 60.01 | 172 | 65 |
100.00%
|
80.00%
|