| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2331 | Thermotoga | GGGGACCGAATTCTCGTGA | CGTGCTTCTTGCTCCGTTG | 59.10 | 59.79 | 158 | 65 |
100.00%
|
80.00%
|
| Primer 2332 | Thermotoga | GGGCTCAGAATCGCGTTGA | AGCACGTTCGTGGTGTGT | 60.45 | 59.81 | 287 | 65 |
100.00%
|
40.00%
|
| Primer 2333 | Thermotoga | TTTCGCTCGACGGCATCA | ATCGTCGAAACCGCACGA | 59.74 | 59.74 | 251 | 65 |
100.00%
|
60.00%
|
| Primer 2334 | Thermotoga | ACGCACCGAACGAAGTGA | TTGCTCTGCTCTGAAGGGC | 59.59 | 60.00 | 168 | 65 |
100.00%
|
80.00%
|
| Primer 2335 | Thermotoga | TCTCCTGCTGGTTCTTGGC | CCCCAGCCAGAAACGATGT | 59.62 | 60.00 | 275 | 65 |
100.00%
|
60.00%
|
| Primer 2336 | Thermotoga | TCTCCTGCTGGTTCTTGGC | ACCCCAGCCAGAAACGATG | 59.62 | 60.00 | 276 | 65 |
100.00%
|
60.00%
|
| Primer 2337 | Thermotoga | TGCACAGGCTCGATTTCGA | CGTACACACCAAGTCCGCT | 59.71 | 60.01 | 204 | 65 |
100.00%
|
80.00%
|
| Primer 2338 | Thermotoga | TCCCTTCAACGGTGGAACG | AGAGCCTTTGCCCATGGAC | 59.93 | 60.00 | 244 | 65 |
100.00%
|
100.00%
|
| Primer 2339 | Thermotoga | TCCCTTCAACGGTGGAACG | CAGAGCCTTTGCCCATGGA | 59.93 | 60.00 | 245 | 65 |
100.00%
|
100.00%
|
| Primer 2340 | Thermotoga | TCCCTTCAACGGTGGAACG | TCAGAGCCTTTGCCCATGG | 59.93 | 60.00 | 246 | 65 |
100.00%
|
100.00%
|