| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2551 | Thermotoga | TTGGGAATCGGCATCGCA | TTCGTCGATGACCGTGCA | 59.73 | 59.35 | 153 | 60 |
100.00%
|
40.00%
|
| Primer 2552 | Thermotoga | GGTCCAAACGGTAGCGGAA | CAGCGGTTTCCTCGAGTTCT | 60.00 | 60.04 | 274 | 60 |
100.00%
|
80.00%
|
| Primer 2553 | Thermotoga | CCGAAGCGAGGGGTTTCAT | ATGAGTGCCTCCGCTGTTC | 60.08 | 60.08 | 229 | 60 |
100.00%
|
80.00%
|
| Primer 2554 | Thermotoga | ATCACGCACACGACGAGAG | CTTGCCCTCGACTGCTTCT | 60.15 | 59.71 | 182 | 60 |
100.00%
|
80.00%
|
| Primer 2555 | Thermotoga | TCCTGTTCTGGGAGTTCGGA | CCAGCTGGCGATTCTCTCT | 60.18 | 59.18 | 170 | 60 |
100.00%
|
80.00%
|
| Primer 2556 | Thermotoga | CCTGTTCTGGGAGTTCGGA | CCAGCTGGCGATTCTCTCT | 58.64 | 59.18 | 169 | 60 |
100.00%
|
80.00%
|
| Primer 2557 | Thermotoga | TGGCGTGATTGATGGTGCA | AAGCCAAGGCAGCTGTCT | 60.30 | 59.16 | 185 | 60 |
100.00%
|
40.00%
|
| Primer 2558 | Thermotoga | GGCGTGATTGATGGTGCAG | AAGCCAAGGCAGCTGTCT | 59.57 | 59.16 | 184 | 60 |
100.00%
|
40.00%
|
| Primer 2559 | Thermotoga | CTGGCGTGATTGATGGTGC | AAGCCAAGGCAGCTGTCT | 59.57 | 59.16 | 186 | 60 |
100.00%
|
40.00%
|
| Primer 2560 | Thermotoga | ACCATTCACACCTGGCCAG | TCTCGTCGTACCTGCCTCT | 59.92 | 59.70 | 157 | 60 |
100.00%
|
100.00%
|