| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2561 | Thermotoga | TGATCACCCGACCGGATGA | TTCCTGCGTGCCCATTCT | 60.38 | 59.24 | 256 | 60 |
100.00%
|
60.00%
|
| Primer 2562 | Thermotoga | TCTGATCACCCGACCGGAT | AAATCGTTCCTGCGTGCC | 60.08 | 58.74 | 264 | 60 |
100.00%
|
40.00%
|
| Primer 2563 | Thermotoga | TTTCTGATCACCCGACCGG | TTCCTGCGTGCCCATTCT | 59.41 | 59.24 | 260 | 60 |
100.00%
|
80.00%
|
| Primer 2564 | Thermotoga | TTCTGATCACCCGACCGGA | AAATCGTTCCTGCGTGCC | 60.30 | 58.74 | 265 | 60 |
100.00%
|
40.00%
|
| Primer 2565 | Thermotoga | TTCGCTGCTGGAAAGGCA | AAGGCGAGCAACCTTGAGG | 59.89 | 60.30 | 196 | 60 |
100.00%
|
80.00%
|
| Primer 2566 | Thermotoga | GGCTGGAAAGGATCGCCAT | GCGTGCATCGAGAAGGTTG | 60.15 | 59.58 | 269 | 60 |
100.00%
|
80.00%
|
| Primer 2567 | Thermotoga | AGCGTTTCACAGGATGGCA | TGAAAGGCCTTCCACGTCC | 59.93 | 59.93 | 180 | 60 |
100.00%
|
60.00%
|
| Primer 2568 | Thermotoga | AGCGTTTCACAGGATGGCA | AAAGGCCTTCCACGTCCTG | 59.93 | 59.93 | 178 | 60 |
100.00%
|
60.00%
|
| Primer 2569 | Thermotoga | ACCCTTCGCTCCTTTGTGG | TTTGAGGATGCGCTCCCTC | 59.93 | 59.78 | 162 | 60 |
100.00%
|
80.00%
|
| Primer 2570 | Thermotoga | TTCAGGCGATGGAAAGCACT | TCTCACCGCTGACTCTGGA | 59.96 | 59.93 | 186 | 60 |
100.00%
|
80.00%
|