| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3351 | Thermotoga | AGCAGTTTCGAAGCCACGA | ACCCCATACCTCCTCGACA | 59.93 | 59.30 | 260 | 42 |
100.00%
|
40.00%
|
| Primer 3352 | Thermotoga | AGCGATCCAATTGCCGACA | CCCTGCTTTCCATCCTCGA | 60.08 | 59.09 | 164 | 42 |
100.00%
|
60.00%
|
| Primer 3353 | Thermotoga | TGGCAGCCTTTGTGACGA | GGCGAAGATACCAGGTGCA | 59.49 | 59.78 | 203 | 42 |
100.00%
|
80.00%
|
| Primer 3354 | Thermotoga | TGGCAGCCTTTGTGACGA | TGGCGAAGATACCAGGTGC | 59.49 | 59.78 | 204 | 42 |
100.00%
|
80.00%
|
| Primer 3355 | Thermotoga | TGGCAGCCTTTGTGACGA | CCACCCCACCGAACATCAT | 59.49 | 59.70 | 168 | 42 |
100.00%
|
60.00%
|
| Primer 3356 | Thermotoga | TTGGGGTGGGAGACGGATT | ACAACGACAACGCTGCGA | 60.54 | 60.58 | 264 | 42 |
100.00%
|
100.00%
|
| Primer 3357 | Thermotoga | TGGAAAGGGCAAGCTGGAG | TGCACGTCACAGCCGTTT | 59.92 | 60.51 | 168 | 42 |
100.00%
|
60.00%
|
| Primer 3358 | Thermotoga | TGGCTTCGACGATCCAACC | TCGTCGATCCACGGCTTT | 60.08 | 59.03 | 288 | 42 |
100.00%
|
60.00%
|
| Primer 3359 | Thermotoga | ATCGGACCACGCAGCTTT | TCGCGAGTCTTGCCATGT | 59.65 | 59.34 | 269 | 42 |
100.00%
|
80.00%
|
| Primer 3360 | Thermotoga | TGGTCAACGTGGACGCAA | CTCCACAAGACACACCGCA | 59.81 | 60.23 | 188 | 41 |
100.00%
|
40.00%
|