| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3371 | Thermotoga | ACGAAAACGGCACCTGGA | TTCGACACACGCTCCGAT | 59.49 | 59.04 | 211 | 40 |
100.00%
|
40.00%
|
| Primer 3372 | Thermotoga | GCGCTTCAGGGAGAATGGT | TCGCAAGGACAGTGGCAA | 60.08 | 59.49 | 293 | 39 |
100.00%
|
60.00%
|
| Primer 3373 | Thermotoga | GCGCTTCAGGGAGAATGGT | TTCGCAAGGACAGTGGCA | 60.08 | 59.49 | 294 | 39 |
100.00%
|
60.00%
|
| Primer 3374 | Thermotoga | AAGGTTCTTCGCGTGGCT | CGTTGGTGGGCTCTGTGAA | 59.57 | 60.23 | 251 | 39 |
100.00%
|
80.00%
|
| Primer 3375 | Thermotoga | TGTGGGTGTACATCCGCAC | ACGCGTCCCTGATGTGAA | 60.00 | 58.94 | 221 | 39 |
100.00%
|
40.00%
|
| Primer 3376 | Thermotoga | CGTGGGTGTGAACCTCGAA | ACGCGTCCCTGATGTGAA | 59.93 | 58.94 | 293 | 39 |
100.00%
|
80.00%
|
| Primer 3377 | Thermotoga | CACATCAGGGACGCGTACA | TGAACGGCTGTGGTGGAA | 59.79 | 59.09 | 256 | 39 |
100.00%
|
80.00%
|
| Primer 3378 | Thermotoga | GGGGACCGAATTCTCGTGA | TCCACCGTGCTTCTTGCT | 59.10 | 59.17 | 163 | 39 |
100.00%
|
60.00%
|
| Primer 3379 | Thermotoga | GCTCGCGAGTATTCTGGCT | GCCACGGTGATGAAGGTGA | 59.93 | 60.00 | 159 | 39 |
100.00%
|
80.00%
|
| Primer 3380 | Thermotoga | AGCACCATTGTGACCGCT | TGCCGTTTGTGGTCGTGA | 59.57 | 59.81 | 214 | 39 |
100.00%
|
80.00%
|