| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3381 | Thermotoga | CTTGCTCAGACCGCTGGAA | TCCTTCCACCTGAACCGTG | 60.00 | 59.25 | 165 | 39 |
100.00%
|
80.00%
|
| Primer 3382 | Thermotoga | TCAGCATCGACCAGGTGT | TGAAGGGCTTTCACCGCT | 58.21 | 59.16 | 265 | 39 |
100.00%
|
40.00%
|
| Primer 3383 | Thermotoga | ATGCGGTGCCTGTGAACT | TCCCGTTCCTCTCTTCGGT | 59.57 | 59.92 | 188 | 39 |
100.00%
|
60.00%
|
| Primer 3384 | Thermotoga | GCGTTCTTTTCGTGGGCT | TTCCATCCCGATTGCCCAC | 58.66 | 60.08 | 198 | 39 |
100.00%
|
40.00%
|
| Primer 3385 | Thermotoga | ACGACACACCGTTCACGAA | ACCGAAGACGTTTCCGCT | 59.86 | 59.27 | 182 | 39 |
100.00%
|
80.00%
|
| Primer 3386 | Thermotoga | TCAGGGTAACGGCTGCAT | ACCCATTCGAAGCTCGCT | 58.60 | 59.02 | 200 | 39 |
100.00%
|
40.00%
|
| Primer 3387 | Thermotoga | ATCAGGGTAACGGCTGCA | ACCCATTCGAAGCTCGCT | 58.60 | 59.02 | 201 | 39 |
100.00%
|
40.00%
|
| Primer 3388 | Thermotoga | AGGTTTGACGGCTCGTTGA | AGAGTCCAGCTTCTCCGGT | 59.56 | 59.92 | 162 | 39 |
100.00%
|
80.00%
|
| Primer 3389 | Thermotoga | CGTTGTGGGAGCAGGGAAT | TCAGAACGGCTGCTGTGT | 60.00 | 59.18 | 290 | 39 |
100.00%
|
80.00%
|
| Primer 3390 | Thermotoga | CTTTGGGAGGGTTCAGGCT | TCGGGAATCCCAGGGTGAT | 59.23 | 60.00 | 193 | 39 |
100.00%
|
40.00%
|