| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3401 | Thermotoga | TGTTCTCTGCACCGGTGA | TGCCACAAAAGCGAGGGT | 58.46 | 59.81 | 274 | 39 |
100.00%
|
60.00%
|
| Primer 3402 | Thermotoga | TTCTCCTCTGAACGTCGCC | TGCCACAAAAGCGAGGGT | 59.41 | 59.81 | 178 | 39 |
100.00%
|
80.00%
|
| Primer 3403 | Thermotoga | ACGCCATCAATGTGGGACT | CTTGGGAAGGAACCGTGCT | 59.31 | 59.93 | 214 | 39 |
100.00%
|
40.00%
|
| Primer 3404 | Thermotoga | CGTCGTGGTGGACCTTGAA | ACGGCTTTCCCAATCGCT | 59.93 | 59.65 | 258 | 39 |
100.00%
|
40.00%
|
| Primer 3405 | Thermotoga | TTCGTCGTGGTGGACCTTG | ACGGCTTTCCCAATCGCT | 59.93 | 59.65 | 260 | 39 |
100.00%
|
40.00%
|
| Primer 3406 | Thermotoga | TCGGGTATCCCTGTGTGGT | ACGCTTCGGTCGCAACT | 59.92 | 59.61 | 224 | 39 |
100.00%
|
80.00%
|
| Primer 3407 | Thermotoga | GCAAGGCCAAAAGACGCT | TCGCAGTGATCGGAGAAGC | 58.96 | 59.86 | 154 | 39 |
100.00%
|
60.00%
|
| Primer 3408 | Thermotoga | GCAAGGCCAAAAGACGCT | CGCAGTGATCGGAGAAGCT | 58.96 | 59.86 | 153 | 39 |
100.00%
|
60.00%
|
| Primer 3409 | Thermotoga | TCGTCGACGAGAACCCTCA | TGCTGTGTTCATGACCGCT | 60.30 | 59.93 | 288 | 39 |
100.00%
|
60.00%
|
| Primer 3410 | Thermotoga | AAGTGGGAGCCGAAGAGGA | ACGTGCTGTTTCTCCTGCT | 60.23 | 59.55 | 262 | 39 |
100.00%
|
80.00%
|