| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3421 | Thermotoga | TGCACTGTTGGTCGCTCT | CAGGCCTGCCAACATAGCT | 59.18 | 60.08 | 232 | 38 |
100.00%
|
40.00%
|
| Primer 3422 | Thermotoga | ATCAAGGAACTGGCACGGG | CAAGGTGAGACCGGTGTCA | 60.00 | 59.25 | 273 | 37 |
100.00%
|
80.00%
|
| Primer 3423 | Thermotoga | AGCAGCGGTGGAATTGCT | TCGCACATCACGATCAGGG | 59.97 | 59.86 | 189 | 37 |
100.00%
|
60.00%
|
| Primer 3424 | Thermotoga | AGATCTTTCGGAGCACGCC | ACCACGCAAGGCCAGAAA | 60.15 | 59.81 | 153 | 37 |
100.00%
|
80.00%
|
| Primer 3425 | Thermotoga | TGCTGGGCAAGGACGAAA | TCAACTGTCTGAGCTCAGGG | 59.49 | 59.03 | 162 | 37 |
100.00%
|
40.00%
|
| Primer 3426 | Thermotoga | TTGCTGGGCAAGGACGAA | TCAACTGTCTGAGCTCAGGG | 59.49 | 59.03 | 163 | 37 |
100.00%
|
40.00%
|
| Primer 3427 | Thermotoga | CGTGGTGGCTCCCGATAAA | ATGGCACCTTCCATCGCA | 59.78 | 59.32 | 264 | 37 |
100.00%
|
80.00%
|
| Primer 3428 | Thermotoga | AGCAGCGGTGGAATTGCT | TCGCACATCACGATCAGGG | 59.97 | 59.86 | 189 | 37 |
100.00%
|
60.00%
|
| Primer 3429 | Thermotoga | TCTTCCTGGAGCTTTCGCC | ACTCGAGACCCTCCTGAACA | 59.70 | 59.89 | 275 | 37 |
100.00%
|
80.00%
|
| Primer 3430 | Thermotoga | AGGTTGCGAGGGAAAAGGG | TGATCCCACGCGTCCTTT | 59.93 | 58.93 | 241 | 37 |
100.00%
|
80.00%
|